@Article{phyton.2020.10022,
AUTHOR = {Lantian Zhang, Siyi Wang, Yuyu Chen, Mengyuan Dong, Yunxia Fang, Xian Zhang, Tao Tong, Ziling Zhang, Junjun Zheng, Dawei Xue, Xiaoqin Zhang},
TITLE = {Genome-Wide Identification of the F-box Gene Family and Expression Analysis under Drought and Salt Stress in Barley},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {89},
YEAR = {2020},
NUMBER = {2},
PAGES = {229--251},
URL = {http://www.techscience.com/phyton/v89n2/38670},
ISSN = {1851-5657},
ABSTRACT = {The F-box protein-encoding gene family plays an essential role in plant
stress resistance. In present study, 126 non-redundant F-box genes were identified
in barley (Hordeum vulgare L., Hv). The corresponding proteins contained 165–
887 amino acid residues and all were amphiphilic, except 5 proteins. Phylogenetic
analysis of F-box protein sequences in barley and stress-related F-box protein
sequences in wheat and Arabidopsis thaliana (At) was used to classify barley
F-box genes are divided into 9 subfamilies (A–I). A structure-based sequence
alignment demonstrated that F-box proteins were highly conserved with a total
of 10 conserved motifs. In total, 124 F-box genes were unevenly distributed on
7 chromosomes; another 2 genes have not been anchored yet. The gene structure
analysis revealed high variability in the number of exons and introns in F-box
genes. Comprehensive analysis of expression profiles and phylogenetic tree analysis, a total of 12 F-box genes that may be related to stress tolerance in barley
were screened. Of the 12 detected F-box genes, 8 and 10 were upregulated
after drought and salt stress treatments, respectively, using quantitative real-time
polymerase chain reaction (qRT-PCR). This study is the first systematic analysis
conducted on the F-box gene family in barley, which is of great importance
for clarifying this family’s bioinformatic characteristics and elucidating its function in barley stress resistance. These results will serve as a theoretical reference
for subsequent research on molecular regulation mechanisms, genetic breeding,
and improvement.},
DOI = {10.32604/phyton.2020.10022}
}