Open Access
ARTICLE
Genome-Wide Identification of the F-box Gene Family and Expression Analysis under Drought and Salt Stress in Barley
Lantian Zhang, Siyi Wang, Yuyu Chen, Mengyuan Dong, Yunxia Fang, Xian Zhang, Tao Tong, Ziling Zhang, Junjun Zheng, Dawei Xue*, Xiaoqin Zhang*
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China
* Corresponding Authors: Dawei Xue. Email: ; Xiaoqin Zhang. Email:
Phyton-International Journal of Experimental Botany 2020, 89(2), 229-251. https://doi.org/10.32604/phyton.2020.10022
Received 05 February 2020; Accepted 29 February 2020; Issue published 22 April 2020
Abstract
The F-box protein-encoding gene family plays an essential role in plant
stress resistance. In present study, 126 non-redundant
F-box genes were identified
in barley (
Hordeum vulgare L.,
Hv). The corresponding proteins contained 165–
887 amino acid residues and all were amphiphilic, except 5 proteins. Phylogenetic
analysis of F-box protein sequences in barley and stress-related F-box protein
sequences in wheat and
Arabidopsis thaliana (
At) was used to classify barley
F-box genes are divided into 9 subfamilies (A–I). A structure-based sequence
alignment demonstrated that F-box proteins were highly conserved with a total
of 10 conserved motifs. In total, 124
F-box genes were unevenly distributed on
7 chromosomes; another 2 genes have not been anchored yet. The gene structure
analysis revealed high variability in the number of exons and introns in
F-box
genes. Comprehensive analysis of expression profiles and phylogenetic tree analysis, a total of 12
F-box genes that may be related to stress tolerance in barley
were screened. Of the 12 detected
F-box genes, 8 and 10 were upregulated
after drought and salt stress treatments, respectively, using quantitative real-time
polymerase chain reaction (qRT-PCR). This study is the first systematic analysis
conducted on the
F-box gene family in barley, which is of great importance
for clarifying this family’s bioinformatic characteristics and elucidating its function in barley stress resistance. These results will serve as a theoretical reference
for subsequent research on molecular regulation mechanisms, genetic breeding,
and improvement.
Keywords
Cite This Article
Zhang, L., Wang, S., Chen, Y., Dong, M., Fang, Y. et al. (2020). Genome-Wide Identification of the F-box Gene Family and Expression Analysis under Drought and Salt Stress in Barley.
Phyton-International Journal of Experimental Botany, 89(2), 229–251. https://doi.org/10.32604/phyton.2020.10022
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