Open Access
ARTICLE
Chromium Differentially Affects Hydrogen Peroxide Distribution in Primary and Adventitious Roots of Arabidopsis thaliana L.
Aarón Giovanni Munguía-Rodríguez1,2, José López-Bucio1, Gerardo Rangel Sánchez1,2, León Francisco Ruiz-Herrera1, Yazmín Carreón-Abud2, Miguel Martínez-Trujillo2,*
1 Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, C. P, 58030, Morelia, Michoacán, México
2 Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo. Edificio R, Ciudad Universitaria, C. P, 58030 Morelia, Michoacán, México
* Corresponding Author: Miguel Martínez-Trujillo. Email:
Phyton-International Journal of Experimental Botany 2020, 89(1), 35-43. https://doi.org/10.32604/phyton.2020.07835
Received 02 July 2019; Accepted 16 October 2019; Issue published 01 March 2020
Abstract
The post-embryonic growth of the
Arabidopsis thaliana root system can
be modified by different types of stress, such as sublethal concentrations of metals,
which may induce the production of reactive oxygen species (ROS). In this study,
the effects of different concentrations of potassium chromate (K
₂CrO
4) on the
distribution and relative quantity of hydrogen peroxide (H
2O
2) were determined in
primary and adventitious roots in
A. thaliana HyPer line seedlings. This line has a
biosensor that specifically reports H
2O
2 levels within tissues as fluorescence.
Primary root growth was inhibited at 100 μM Cr (VI); in contrast, adventitious root
formation was induced over the main root growth axis. These structures
proliferated from 100-160 μM Cr (VI), and much higher concentrations (180-200
μM) of K
₂CrO
4 were required to affect their growth. The H
2O
2 distributions were
observed in the columella and lateral root cap of primary roots of plants grown in
medium lacking dichromate, but following the development of toxicity symptoms, H
2O
2 changed its distribution to the meristem and differentiation zones. Conversely,
adventitious roots had comparable H
2O
2 distribution patterns in untreated plants
and those exposed to Cr (VI) supplementation. Thus, differential H
2O
2 distribution
correlates with the resistance of adventitious roots, but not primary roots, to
dichromate and underlies cell reorganization at the apex to support growth.
Keywords
Cite This Article
Munguía-Rodríguez, A. G., López-Bucio, J., Sánchez, G. R., Ruiz-Herrera, L. F., Carreón-Abud, Y. et al. (2020). Chromium Differentially Affects Hydrogen Peroxide Distribution in Primary and Adventitious Roots of
Arabidopsis thaliana L..
Phyton-International Journal of Experimental Botany, 89(1), 35–43. https://doi.org/10.32604/phyton.2020.07835