Open Access
ARTICLE
Exogenous Spermidine Promotes Somatic Embryogenesis of Cunninghamia lanceolata by Altering the Endogenous Phytohormone Content
Dandan Wang1,#, Yulin Guo1,#, Xiaofei Long1, Yan Pan1, Dingjie Yang1, Rui Li1, Ye Lu1, Ying Chen1,2, Jisen Shi1, Jinhui Chen1,*
1 Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China
2 Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement Nanjing Forestry University, Nanjing, 210037, China
# These authors contributed equally: Dandan Wang, Yulin Guo
* Corresponding Author: Jinhui Chen. Email:
Phyton-International Journal of Experimental Botany 2020, 89(1), 27-34. https://doi.org/10.32604/phyton.2020.08971
Received 29 October 2019; Accepted 13 November 2019; Issue published 01 March 2020
Abstract
In order to study how exogenous hormones in
C. lanceolata
(gymnosperm) regulate somatic embryogenesis, we measured the endogenous
phytohormones of two genotypes with different somatic embryogenesis
efficiency and found that an increase in endogenous concentrations of IAA and
ABA may be correlated to more efficient somatic embryogenesis. By applying
exogenous spermidine, we found that exogenous hormones may affect somatic
embryogenesis efficiency through affecting the endogenous phytohormone
content. Based on these results, further studies can be conducted whereby the
concentration of exogenous hormones or the levels of endogenous
phytohormones by molecular methods are regulated to promote somatic
embryogenesis. Our research may benefit the long-term economic output of the
forestry industry and lays the foundation to studying the molecular mechanism
that controls somatic embryogenesis efficiency.
Keywords
Cite This Article
APA Style
Wang, D., Guo, Y., Long, X., Pan, Y., Yang, D. et al. (2020). Exogenous spermidine promotes somatic embryogenesis of cunninghamia lanceolata by altering the endogenous phytohormone content. Phyton-International Journal of Experimental Botany, 89(1), 27-34. https://doi.org/10.32604/phyton.2020.08971
Vancouver Style
Wang D, Guo Y, Long X, Pan Y, Yang D, Li R, et al. Exogenous spermidine promotes somatic embryogenesis of cunninghamia lanceolata by altering the endogenous phytohormone content. Phyton-Int J Exp Bot. 2020;89(1):27-34 https://doi.org/10.32604/phyton.2020.08971
IEEE Style
D. Wang et al., "Exogenous Spermidine Promotes Somatic Embryogenesis of Cunninghamia lanceolata by Altering the Endogenous Phytohormone Content," Phyton-Int. J. Exp. Bot., vol. 89, no. 1, pp. 27-34. 2020. https://doi.org/10.32604/phyton.2020.08971
Citations