Open Access

ARTICLE

Exogenous Spermidine Promotes Somatic Embryogenesis of Cunninghamia lanceolata by Altering the Endogenous Phytohormone Content

Dandan Wang^1,#, Yulin Guo^1,#, Xiaofei Long¹, Yan Pan¹, Dingjie Yang¹, Rui Li¹, Ye Lu¹, Ying Chen^1,2, Jisen Shi¹, Jinhui Chen^1,*
1 Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China
2 Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement Nanjing Forestry University, Nanjing, 210037, China
# These authors contributed equally: Dandan Wang, Yulin Guo
* Corresponding Author: Jinhui Chen. Email:

Phyton-International Journal of Experimental Botany 2020, 89(1), 27-34. https://doi.org/10.32604/phyton.2020.08971

Received 29 October 2019; Accepted 13 November 2019; Issue published 01 March 2020

Abstract

In order to study how exogenous hormones in C. lanceolata (gymnosperm) regulate somatic embryogenesis, we measured the endogenous phytohormones of two genotypes with different somatic embryogenesis efficiency and found that an increase in endogenous concentrations of IAA and ABA may be correlated to more efficient somatic embryogenesis. By applying exogenous spermidine, we found that exogenous hormones may affect somatic embryogenesis efficiency through affecting the endogenous phytohormone content. Based on these results, further studies can be conducted whereby the concentration of exogenous hormones or the levels of endogenous phytohormones by molecular methods are regulated to promote somatic embryogenesis. Our research may benefit the long-term economic output of the forestry industry and lays the foundation to studying the molecular mechanism that controls somatic embryogenesis efficiency.

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Keywords

Spermidine; C. lanceolata; somatic embryogenesis; endogenous phytohormones

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Citations