Open Access

ARTICLE

Morphometric and Biochemical Changes in Agave americana L. Plantlets Induced By Ethyl Methanesulfonate

S. J. Reyes-Zambrano^1,†, M. L. Ramírez-Merchant^1,†, C. Arias-Castro², M. A. Rodríguez-Mendiola², C. A. Lecona-Guzmán¹, V. M. Ruíz-Valdiviezo¹, D. González-Mendoza³, F. A. Gutiérrez-Miceli^1,*

1 Laboratorio de Biotecnología Vegetal, Instituto Tecnológico de Tuxtla Gutiérrez, Tecnológico Nacional de México. Carretera Panamericana Km. 1080, Terán, Tuxtla Gutiérrez, Chiapas, C.P. 29050, México.
2 Laboratorios de Análisis Instrumental Bioquímico, DEPI del Instituto Tecnológico de Tlajomulco, Tecnológico Nacional de México. Tlajomulco de Zuñiga, C.P. 45640, México.
3 Instituto de Ciencias Agrícolas, Universidad Autónoma de Baja California (ICA-UABC), Carretera a Delta s/n Ejido Nuevo León, CP.21705, Baja California, México.
† Both authors contributed in the same way in the realization of the experimental work.
* Corresponding Author: F. A. Gutiérrez-Miceli. Email: fgmiceli@gmail.com.

Phyton-International Journal of Experimental Botany 2019, 88(3), 277-284. https://doi.org/10.32604/phyton.2019.06504

Abstract

A. americana L. is a crop with very little genetic variability. In order to evaluate the effect of ethyl methanesulfonate (EMS) to induce variability in in vitro plantlets of A. americana, different explants (meristems, leaves and roots) were evaluated for the production of callus. MS medium supplemented with ANA (2.68 μM) and BAP (2.68 μM) was used. Callus obtained from apical meristem were treated with 15 mM EMS for two hours after which shoot formation was induced using 2,4-D (0.11 μM) and BAP (44 μM). The EMS induced variations in the morphometric and morphological parameters of the plantlets obtained, with 60% of the plantlets presenting differences such as dwarfism and different leaf forms, without the presence of spines, as well as an increase in fructan content of 30% with respect to the control plantlets. PAL was increased and this activity is related with higher anthocyanins concentration in A. americana L. plantlets.

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Keywords

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