Open Access

ARTICLE

Total Phenols, Flavonoids and Antioxidant Activity in Annona muricata and Annona purpurea Callus Culture

by M. Y. Ovando-Domínguez, M. C. Luján-Hidalgo, D. González-Mendoza, A. A. Vargas-Díaz, N. Ruiz-Lau, F. A. Gutiérrez-Miceli, C. A. Lecona-Guzmán

1 Laboratorio de Cultivo de Tejidos Vegetales. Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana Km. 1080. Tuxtla Gutiérrez, Chiapas, C.P. 29050, México.
2 Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California (ICA-UABC). Carretera a Delta s/n C.P. 21705, Ejido Nuevo León, Baja California, México.
3 CONACYT-Colegio de Postgraduados, Campus Campeche, Carretera Federal Haltunchén-Edzna km 17.5. Sihochac, Champotón, Campeche, CP 24450, México.
4 CONACYT-Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez. Carretera Panamericana Km. 1080. Tuxtla Gutiérrez, Chiapas, C.P. 03940, México.

* Corresponding Author: C. A. Lecona-Guzmán. Email: .

Phyton-International Journal of Experimental Botany 2019, 88(2), 139-147. https://doi.org/10.32604/phyton.2019.06546

Abstract

Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog (MS) medium supplemented with different concentrations of 1-naphthylacetic acid (NAA), 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) utilized hypocotyls with explant. The highest percentage of callus formation was the treatment supplemented with 3 mg L^-1 NAA for A. muricata (100%) while for A. purpurea in lower percentage (75%). BA stimulated the formation of shoots in all the evaluated concentrations, being the concentration of 2 mg L^-1 the one that induced the greater formation of shoots for A. muricata (23 shoots/explant) and A. purpurea (28 shoots/explant). The content of total phenols, flavonoids and antioxidant activity was measured in the callus obtained from both species. The results showed that a higher content of total phenols was quantified in callus of A. purpurea (27.8 mg g^-1 dw) compared to A. muricata (23.2 mg g^-1 dw). The highest content of total flavonoids was observed in the callus of A. purpurea (8.0 μg g^-1 dw). Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydracil radical assay. The concentration required for 50% inhibition (IC50) of the 2,2-diphenyl-1-picrylhydracil radicals were 4.22 μg mL^-1 in methanolic extracts of callus of A. muricata, while in extracts of callus of A. purpurea was 2.86 μg mL^-1, in both cases was greater than that found for leaves. Callus culture of the species studied in this work represents an alternative for the production of natural antioxidants.

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