Open Access

ARTICLE

Torin 1, TOR Inhibitor Enhances Cellular Proliferation in NT-1 Tobacco Suspension Cell Cultures

Grisel Fierros-Romero^1,*, M. Elena Mellado-Rojas², Reynaldo C. Pless³, Juan Ignacio Valiente-Banuet¹, Ashutosh Sharma¹ and Elda M. Beltrán-Peña²

1 Tecnológico de Monterrey, School of Engineering and Sciences, Campus Querétaro, 76130, Querétaro, México.
2 Instituto de Investigaciones Químico Biológicas. Universidad Michoacana de San Nicolás de Hidalgo, 58030. Morelia, México.
3 CICATA-Instituto Politécnico Nacional, Querétaro, 76090, México.

Phyton-International Journal of Experimental Botany 2019, 88(2), 131-137. https://doi.org/10.32604/phyton.2019.05036

Abstract

Torin 1 is an ATP-competitive TOR inhibitor which inhibits the signaling of TOR and S6K kinase in mammals and plants. The objective of this research is to determine the effect of Torin 1 in a relatively simple and homogeneous plant system such as the NT-1 tobacco suspension cell cultures. Cultures of NT-1 cells were tested with 5, 50, 150 and 250 nM of Torin 1. During kinetics growth of NT-1 tobacco suspension cell cultures, 150 and 250 nM Torin 1 inhibits the early growth and later enhanced the cellular proliferation during exponential growth by means of an increased expression of E2F1 and cyclin B. Furthermore, Torin 1 stimulates the growth of NT-1 cells during log phase with small shaped cell, characteristic of tobacco suspension cell cultures with high mitotic activity.

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