Open Access
ARTICLE
Effects of Rhizoglomus intraradices, Azospirillum brasilense and plant growth regulators application on root architecture in barley (Hordeum vulgare L.)
Zepeda-Guzmán S1, M Gómez-Romero2, C Sosa-Aguirre1, J Villegas1
1 Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Mújica s/n, Colonia Felícitas del Rio, C.P. 58030, Morelia,
Michoacán, México.
2 Cátedras CONACYT-Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Mújica s/n, Colonia Felícitas del Rio, C.P. 58030, Morelia,
Michoacán, México.
Address correspondence to: Dr. Javier Villegas, Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Mujica s/n, Colonia Felícitas del Rio, C.P. 58030, Morelia, Michoacán, México, e-mail: vilj4455@yahoo.com.mx
Phyton-International Journal of Experimental Botany 2018, 87(all), 183-190. https://doi.org/10.32604/phyton.2018.87.183
Abstract
Changes in root architecture are a strategy used by
plants to explore the soil for available resources. The presence of beneficial
microorganisms in the rhizosphere as well as plant growth
regulators can cause changes in root development and promote the
availability of water and nutrients. The effect of microorganisms or
growth regulators on plant growth has been tested, but little is known
about the effect they have on the architecture of the root of
Hordeum
vulgare L. Therefore the objective of this study was to evaluate the
effect of the application of
Rhizoglomus intraradices, Azospirillum
brasilense, quercetin and epibrassinolide, alone or in combination,
on the root architecture and plant (shoot + root) biomass of barley.
The experiment was conducted in a growth chamber and a rhizotron
system was used as a case study to assess the root architecture. Each
of the barley seeds that germinated in the rhizotrons was inoculated
with 70 spores of
R. intraradices or 2.5 x 10
7 CFU/mL of A.
brasilense in a 1 mL suspension. Quercetin and/or epibrassinolide were
applied in 5 mL of solution at a concentration of 10 μM, at 0, 5 and
8 days. The results showed that the growth promoter epibrassinolide
affected barley root architecture by increasing the number, but
decreasing the length, of seminal roots. It also promoted the early
onset of lateral roots. Quercitin, applied alone or combined, had a
significant effect to increase the number of lateral roots. Although
changes were observed in the architecture of the root, barley biomass
did not show significant differences in this evaluation period. The
application of microorganisms did not produce significant changes
in the variables evaluated.
Keywords
Cite This Article
APA Style
S, Z., Gómez-Romero, M., Sosa-Aguirre, C., Villegas, J. (2018). Effects of rhizoglomus intraradices, azospirillum brasilense and plant growth regulators application on root architecture in barley (hordeum vulgare L.). Phyton-International Journal of Experimental Botany, 87(all), 183-190. https://doi.org/10.32604/phyton.2018.87.183
Vancouver Style
S Z, Gómez-Romero M, Sosa-Aguirre C, Villegas J. Effects of rhizoglomus intraradices, azospirillum brasilense and plant growth regulators application on root architecture in barley (hordeum vulgare L.). Phyton-Int J Exp Bot. 2018;87(all):183-190 https://doi.org/10.32604/phyton.2018.87.183
IEEE Style
Z. S, M. Gómez-Romero, C. Sosa-Aguirre, and J. Villegas "Effects of Rhizoglomus intraradices, Azospirillum brasilense and plant growth regulators application on root architecture in barley (Hordeum vulgare L.)," Phyton-Int. J. Exp. Bot., vol. 87, no. all, pp. 183-190. 2018. https://doi.org/10.32604/phyton.2018.87.183