Open Access

ARTICLE

Genetic diversity of water primrose (Ludwigia hyssopifolia) in Thailand based on morphological characters and RAPD analysis

by Tantasawat PA, K Lunwongsa, T Linthaisong, P Wirikitgul, N Campatong, N Talpolkrung, A Tharapreuksapong, O Poolsawat, A Khairum, A Sorntip, C Kativat

Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand.

Address correspondence to: Piyada Alisha Tantasawat, e-mail:

Phyton-International Journal of Experimental Botany 2018, 87(all), 162-170. https://doi.org/10.32604/phyton.2018.87.162

Abstract

Genetic diversity and relatedness of 17 water primrose (Ludwigia hyssopifolia) accessions in Thailand were estimated using morphological characters and random amplified polymorphic DNA (RAPD) markers. Eight morphological characters were diverse among the accessions. However, some accessions could not be distinguished from one another based on these morphological characters alone. Unweighted pair-group arithmetic average (UPGMA) analysis of these characters separated these 17 accessions into 2 major clusters. Among the 5 RAPD primers used, a total of 68 fragments (150 to 2000 bp) were amplified, showing a polymorphism percentage of 80%. The polymorphic information content (PIC) among accessions varied from 0.31 to 0.36 with an average of 0.33. These polymorphic RAPD markers successfully distinguished all 17 water primrose accessions. The 17 accessions were grouped into 3 clusters using UPGMA analysis. Pairwise coefficients of morphological characters and RAPD-based genetic similarity between all accessions ranged from 0.154 to 1.000 with an average of 0.693 and from 0.531 to 0.952 with an average of 0.802, respectively. A nonsignificant correlation between morphological characters and RAPD-based similarities was found as shown by the low correlation coefficient of 0.242 between the two Jaccard’s similarity matrices in the Mantel test. Clustering of accessions within clusters also differed when morphological characters and RAPD-derived dendrograms from UPGMA analysis were compared. It would suggest that RAPD was more effective in determining the genetic variability and relationships among water primrose accessions. In addition, RAPD was also more useful for accessions identification since all 17 water primrose accessions can be effectively distinguished by only 5 RAPD primers.

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