Open Access

ARTICLE

Molecular differentiation of Xoconostle (Opuntia spp.) using AFLP molecular markers

by García-Zambrano EA, MC Ojeda-Zacarías, JG Gutiérrez-Soto, SR Sinagawa-García, A Gutiérrez-Díez, J Martínez-de la Cerda, JA Pedroza-Flores, C Gallegos-Vázquez, JI García-Zambrano

1 Facultad de Agronomía. Universidad Autónoma de Nuevo León. Campus de Ciencias Agropecuarias, Francisco Villa s/n. General Escobedo Nuevo León, México.
2 Universidad Autónoma de Chapingo, Centro Regional Universitario Centro Norte. Km. 5,5 Carretera Zacatecas-Guadalajara, El Orito, Zacatecas, México.
3 Instituto Tecnológico y de Estudios Superiores de Monterrey. Campus Monterrey. Ave. Eugenio Garza Sada 2501, Col. Tecnológico, Monterrey, Nuevo León, México.

Address correspondence to: Eduardo Alejandro García-Zambrano, e-mail: ;

Phyton-International Journal of Experimental Botany 2018, 87(all), 87-93. https://doi.org/10.32604/phyton.2018.87.087

Abstract

The technologies based on molecular markers of DNA are the most advanced and possibly the most effective and accurate means to identify genetic variation. The objective of this study was to use molecular genetic markers namely AFLP as a tool for the molecular differentiation of 36 accessions of xoconostle collected in the Centro Regional Universitario Centro Norte of the Universidad Autónoma de Chapingo (CRUCEN-UACh.). DNA extraction was performed by the method of cellular ruptor using FastDNA^® Kit, and quantification of purity and yield of DNA was carried out through the Nanodrop 2000. It was observed that the DNA concentrations ranged from 108.4 ng/μL to 38.5 ng/μL. Two hundred and ninety five molecular markers were generated with a molecular weight ranging from 50 to 800 bp. The average diversity index (ID) of the 36 accessions was 0.8124. A binary matrix was generated which was analyzed through clusters by the unweighted pair clustering method (UPGMA). In the dendogram two groups of 5 and 31 accessions were generated and the last group was divided into two subgroups consisting of 8 and 23 accessions, respectively. It was observed that there were differences between the accessions of xoconostle, presenting a high degree of similarity because its grouping was realized at short distances and in a staggered way. The accessions Orito Xoconostle 14, Orito Xoconostle 16 had a zero euclidean distance, being considered duplicate, so that only one of them should be established inside the germplasm bank.

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Keywords

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