Open Access
ARTICLE
Species of Trichoderma antagonistic to the root knot nematode (Meloidogyne incognita) in habanero pepper
Herrera-Parra E1,2, J Ramos-Zapata1, J Cristóbal-Alejo3, J Tun-Suarez3, A Reyes-Ramírez3
1
Departamento de Ecología Tropical, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Km. 15.5 Carretera Mérida-Xmatkuil, Mérida, Yucatán, México. C.P. 97315 (jaramos.zapata@gmail.com) (elian.herrera09@gmail.com).
2
Campo Experimental Mocochá. INIFAP. Km. 25 Carretera Antigua a Mérida-Motul, Mocochá, Yucatán, México. C.P. 97450 (herrera.elizabeth@inifap.gob.mx).
3
División de Estudios de Posgrado e Investigación. Instituto Tecnológico de Conkal. C.P. 97345. Conkal, Yucatán, México. Telephone and fax. 52 (999) 9 87 91 35 (jairoca54@hotmail.com.mx).
Address correspondence to: Jairo Cristóbal-Alejo, Conkal, Yucatán, México. C.P. 97345, e-mail:
Phyton-International Journal of Experimental Botany 2018, 87(all), 7-13. https://doi.org/10.32604/phyton.2018.87.007
Abstract
The root knot nematode
Meloidogyne spp. is an important
endoparasite limiting the cultivation of horticultural species
and affecting the plants of at least 3000 species, including grasses,
vegetables, ornamentals, fruit and forest. The aim of this study was
to estimate the potential of three species of
Trichoderma as antagonists
of
M. incognita in plants of
Capsicum chinense under greenhouse
conditions. A bioassay was established with plants of
C. chinense previously
inoculated with
Trichoderma spp. and transplanted to pots
with sterile substrate; the plants were subsequently inoculated with
300 infective second-stage larvae (J
2) and 1000 larvae eggs of
M.
incognita. A completely randomized experimental design was implemented
with six treatments (
T. harzianum-C1,
T. atroviride, T. virens,
T. harzianum-C2, nematicide oxamyl 24% SL and a control)
with 10 replicates for each treatment. After 60 days, the following
parameters were recorded: number of galls per root, galling index,
number of eggs and number of females per gram of root, as well as,
plant height, dry shoot biomass, volume, length and fresh weight of
root. In comparison with the control, the plants inoculated with the
Trichoderma species and those which received the application of the
nematicide oxamyl presented a reduction of 68.18% and 65.07%, respectively,
in the number of galls per root, a galling index of 82.91%
and 70.25%, respectively, number of eggs per gram of root 82.69%
and 77.16%, respectively, and number of females per gram of root
up to 50%. The fungal species studied in this work have potential as
antagonistic agents against
M. incognita, given that they present the
same capacity as the nematicide oxamyl to reduce the galling index
and reproduction of the nematode.
Keywords
Cite This Article
E, H., Ramos-Zapata, J., Cristóbal-Alejo, J., Tun-Suarez, J., Reyes-Ramírez, A. (2018). Species of
Trichoderma antagonistic to the root knot nematode (
Meloidogyne incognita) in habanero pepper.
Phyton-International Journal of Experimental Botany, 87(all), 7–13.