Open Access

ARTICLE

Comparative proteome analysis in hot pepper (Capsicum annuum L.) after space flight

Xie LB¹, X Wang¹, M Peng², Y Zhou¹, LX Chen¹, LX Liu³, YL Gao¹, YH Guo¹

1 Horticultural Sub-Academy, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150069, China.
2 College of Life Science, Northeast Forestry University, Harbin, Heilongjiang, 150040, China.
3 Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China.

Address correspondence to: Luxiang Liu, Tel: +86 451 82191755; Fax: +86 451 86433905, e-mail:

Phyton-International Journal of Experimental Botany 2017, 86(all), 236-245. https://doi.org/10.32604/phyton.2017.86.236

Abstract

Hot pepper (Capsicum annuum L.) is an important crop all over the world. To explore and identify differentially expressed proteins of hot pepper after space flight, three space-induced mutants (Y1, Y2 and Y3), which obtained new traits after space flight compared with their control lines (W1 and W2), were analyzed using comparative proteome analysis. In this study, leaf morphological characteristics of five kinds of hot pepper variations were evaluated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results showed that obvious changes of cellular structure were seen in space induced mutants. Thirty nine out of the 255 differentially expressed proteins were successfully sequenced. Among these 39 proteins, 31 were homologous with known proteins, and the others were set as a hypothetical protein. All identified proteins were further classified into six groups including protein metabolism, energy metabolism and photosynthesis. In conclusion, these findings will benefit for the research of mutagenic mechanisms at the level of proteomics, and provide an effective way for new hot pepper breeding.

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