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ARTICLE

In vitro antagonism of Trichoderma harzianum on Sclerotium cepivorum Berk. and S. rolfsii Sacc., causal agents of onion rot

by Zúñiga-Mendoza E, LF Ceja-Torres

Instituto Politécnico Nacional, CIIDIR-Unidad-Michoacán, Justo Sierra No. 28, Col. Centro, C. P. 59510, Jiquilpan Michoacán, México.

Address correspondence to: Luis Fernando Ceja Torres, e-mail:

Phyton-International Journal of Experimental Botany 2017, 86(all), 7-13. https://doi.org/10.32604/phyton.2017.86.007

Abstract

In vitro tests were carried out, to evaluate the antagonist capacity of a strain of Trichoderma isolated from soil in La Cienega de Chapala, Michoacán, Mexico against Sclerotium cepivorum and Sclerotium rolfsii, casual agents of onion rot, an important disease in Mexico and the rest of the world. Percentages of radial growth inhibition (PRGI) were calculated every 24 h, until a rate of antagonism was obtained according to the Bell’s scale, as well as the percentages of inhibition of the production of sclerotia and their parasitism. The PRGI was 17.4% against S. rolfsii and 22.2% against S. cepivorum after 48 and 72 h, respectively. Trichoderma harzianum obtained a degree of antagonism of 1 on S. cepivorum and two against S. rolfsii, on the Bell scale. On the other hand, T. harzianum reduced (P≤0.05) 95% the production of sclerotia of S. cepivorum and 78% that of S. rolfsii; the parasitism of the sclerotia of S. cepivorum and S. rolfsii was 91% and 54%, respectively.

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