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SCAR molecular markers to identify disease resistance sources in ayocote beans (Phaseolus coccineus)
Centro de Biotecnología Genómica Instituto Politécnico Nacional (CBG-IPN); Boulevard del Maestro, Esq. Elias Piña s/n Reynosa, Tamaulipas, C.P 88710, México.
Instituto Nacional de Investigaciones Agrícolas y Pecuarias; Programa de Recursos Genéticos. Campo Experimental Valle de México. INIFAP. Carretera Los Reyes-Texcoco, km 13.5. A.P. 307. C.P. 56250. Coatlinchán, Texcoco, Estado de México. México.
División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco; Carretera Villahermosa-Cárdenas, entronque Bosques de Saloya, kilómetro 0.5, Villahermosa, Tabasco, C.P. 86040, tel. 993-3-54-43-08. México.
Centro Interdisciplinario De Investigación para el Desarrollo Integral Regional-Unidad Durango, Instituto Politécnico Nacional (CIIDIR-Durango); Calle Sigma #119, Fracc. 20 de Noviembre II, Durango Dgo., C.P. 34220, México.
Universidad México Americana del Norte AC. Primera s/n El Círculo, 88640, Reynosa, México.
Address correspondence to: M.C. Régulo Ruíz-Salazar, e-mail:
Phyton-International Journal of Experimental Botany 2016, 85(all), 184-193. https://doi.org/10.32604/phyton.2016.85.184
Abstract
In order to identify genes of resistance to four diseases that affect Phaseolus sp. [i.e, Common Bacterial Blight (CBB), Bean Common Mosaic Virus (BCMV), Bean Golden Mosaic Virus (BGMV) and angular leaf spot (ALS)], we amplified nine SCAR (Sequence Characterized Amplified Regions) markers: four linked to resistance to common blight, two for BCMV, two for BGMV and one for ALS. The genetic material included 16 ayocote bean (P. coccineus) populations collected from different locations at Veracruz and Puebla, Mexico. Populations from Veracruz showed greater number of SCARs for BGMV and ALS compared with populations from Puebla. The best P. coccineus populations were Jalapa, Veracruz and Acultzingo (Veracruz); Nauzontla, and Zacapoaxtla (Puebla). Cluster analysis separated the bean populations evaluated by geographical origin, but mainly by the presence of two of the nine amplified markers (SU91 and LG5). The study populations, mainly those of Veracruz, should be used in breeding programs to generate segregating populations with genetic resistance to the major diseases of beans in Mexico, since they showed high frequencies of SCARs.Keywords
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