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ARTICLE

Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant

Guan Z-J^1,3,4*, S-B Lu^2*,Y-L Huo⁵, B Guo³, Z-P Guan⁶, Y-H Wei³

1 Department of Life Sciences, Yuncheng University, Yuncheng, Shanxi, 044000, P.R. China.
2 College of life sciences, Jiangxi Normal University, Nanchang, Jiangxi, 330022, P.R. China.
3 Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an, Shannxi, 710069, P.R. China.
4 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
5 Centre of Biological and Chemical Experiment, Yuncheng University, Yuncheng, Shanxi, 044000, China.
6 College of Food Science, Shanxi Normal University, Linfen 04100, China.
* Guan Z-J and S-B Lu contributed equally to this paper.

* Corresponding Authors:Address Correspondence to: Zheng-Jun Guan, Ph. D, e-mail: ; ; Y-H. Wei, Ph D, e-mail:

Phyton-International Journal of Experimental Botany 2015, 84(2), 351-357. https://doi.org/10.32604/phyton.2015.84.351

Abstract

Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of the mutant increased steadily until 42 days from culturing and then decreased sharply. Malondialdehyde (MDA) of the mutant showed a significant decreasing trend after 21 days from culturing. Growth rate of the mutant was at times lower than that of the control during its callus differentiation, and the soluble protein content of the mutant callus decreased from explant cultivation until the 28th day of culture. The mutant had greater values of chlorophyll a, carotenoid and Chlorophyll contents than the control after 14 days of culturing, and Chlorophyll b content of the mutant showed a declining trend. The electrical conductivity trend of the mutant was consistent with that in the control. It indicated that in terms of the organogenesis or somatic embryogenesis pattern, protein synthesis and catabolism were very active, and a number of antioxidant enzyme activities were consistent in the early development stages of the two regeneration systems. These findings were useful for the regeneration of the mutant.

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