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ARTICLE

First morphogenetic identification of Fusarium solani isolated from orange fruit in Egypt

Abd-Elsalam KA^1,2, K Youssef^1,2, H Almoammar³

1 Plant Pathology Research Institute, Agricultural Research Centre, Giza, Egypt.
2 Unit of Excellence in Nano-Molecular Plant Pathology Research, Giza, Egypt.
3 National Centre for Biotechnology, King Abdulaziz City for Science and Technology, Saudi Arabia.

* Corresponding Author:Address Correspondence to: Kamel A. Abd-Elsalam, e-mail:

Phyton-International Journal of Experimental Botany 2015, 84(1), 128-131. https://doi.org/10.32604/phyton.2015.84.128

Abstract

Losses due to postharvest decay may occur at any time during postharvest handling, from harvest to consumption affecting the produce quality and quantity. Accurate identification of the pathogen causing postharvest disease is essential to the selection of an appropriate disease control approach. Nine isolates of Fusarium recovered from orange fruit were identified as Fusarium solani. The fungus is involved with fruit decay. The obtained cultures were purified and grown on potato-dextrose agar (PDA), malt yeast agar (MYA), and Czapek's nutrient media (CNM) under light for identification. A pathogenicity test was carried out to fulfil Koch's postulates. The pathogen could only enter ripe orange fruit through wounds and cracks causing the rot disease. The identification of the fungal isolates was confirmed to be F. solani by DNA sequencing, which was 99 to 100% homologous to those deposited in the Gen- Bank. The identity of nine fungal isolates was confirmed to be F. solani by DNA sequencing of the internal transcribed spacer (ITS) rDNA region (GenBank Accession Nos. DQ486874 to DQ486881 and KC758879). To our knowledge, this is the first morphogenetic identification of F. solani isolated from orange fruit in Egypt.

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