Open Access

ARTICLE

Factors influencing in the response of Schizolobium parahybum (Vell) Blake to Ceratocystis paradoxa and C. moniliformis

by Mora-Silva W¹, FR Garcés-Fiallos¹, C Suarez-Capello¹, CE Belezaca-Pinargote¹, P Cedeño-Loja¹, E Vallejo³

1 Laboratorio de Microbiología Ambiental y Vegetal, Dirección de Investigación Científica y Tecnológica-DICYT, Universidad Técnica Estatal de Quevedo-UTEQ
2 Escuela de Ingeniería Agronómica, Facultad de Ciencias Agrarias, UTEQ .
3 Escuela de Ingeniería Forestal, Facultad de Ciencias Ambientales, UTEQ. Avenida Walter Andrade, Km 1½ vía a Santo Domingo. Casilla Postal: 73. Quevedo, Los Ríos, Ecuador.
Address Correspondence to: Felipe R. Garcés-Fiallos, Escuela de Ingeniería Agronómica, Facultad de Ciencias Agrarias, UTEQ. Avenida Walter Andrade, Km 1½ vía a Santo Domingo. Casilla Postal: 73. Quevedo, Los Ríos, Ecuador, e-mail: felipegarces23@yahoo.com; fern2010libra_ata@hotmail.com; suarezcapello@yahoo.com; cbelezaca@yahoo.com; emilioloja@hotmail.com; eliasvallejoz@hotmail.com

Phyton-International Journal of Experimental Botany 2015, 84(1), 120-127. https://doi.org/10.32604/phyton.2015.84.120

Abstract

In the Ecuadorian coast one of the most destructive diseases of the pachaco is vascular wilt or stem rot caused by Ceratocystis complex, so the aim of this study was to determine the factors that affect the efficiency of the reaction of bark pachaco to this disease. This research was conducted under laboratory conditions, using trees pachaco S38, S41, S98, AE-1, AE-2 and AE-3, and pathogenic species Ceratocystis paradoxa and C. moniliformis. The method utilized was tissue stem bark,with bark sections with 4.5 cm², and a suspension of 3x10⁴ units infection and remained in a humid chamber for 96 hours at 25 ± 5 °C. Were determined grades of resistance/ susceptibility using a scale from 0 to 4, depending on the amount of mycelia and peritecio in each plant sample. Three factors were used: four colonies obtained by several transfers from each fungal specie, four ages of colonies of each fungal specie and four volumes of inoculum applied (units of infection), using for each experiment separately Completely Randomized Design with 4 replications factorial arrangement. For comparison between treatment means was used Tukey test at 5% probability of error. For future trials using this technique, you could use 30-day colonies for C. paradoxa and 40 days for C. moniliformis, and an application volume of 100 μL/cm², it would improve the level of response for the formation of perithecium and mycelia in samples cortex.

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