Open Access

ARTICLE

Comparison of four DNA extraction methods on various tissues and types of Sechium edule ( Jacq.) Sw.

by Galindo-Tovar ME, VS Gasperin-Sanatta, JA Alejandre-Rosas, OR Leyva-Ovalle, J Murguía-González, HE Lee-Espinosa, I L,ero-Torres

Facultad de Ciencias Biológicas y Agropecuarias. Universidad Veracruzana. Camino Peñuela-Amatlán de los Reyes s/n. CP 94945. Tel. (55) 2717166129, Fax (55) 2717166410, Veracruz, México.

* Corresponding Author:Address Correspondence to: María Elena Galindo Tovar, e-mail:

Phyton-International Journal of Experimental Botany 2014, 83(all), 307-310. https://doi.org/10.32604/phyton.2014.83.307

Abstract

Sechium edule is an important crop in Southern Mexico and Central America, and México was the main producer, with 53% of the world production in 2012. This species presents high levels of morphological variation that have resulted in different types of varieties not clearly defined. However, morphological characteristics have not been sufficient to classify S. edule varieties, and it has been difficult to study the species genetic diversity. Because of it, there is a need to develop methods for studying the genetic diversity in S. edule. In this regard, the first step is to find a suitable DNA extraction method. For this reason, in this study four DNA extraction methods (two laboratory protocols, and two commercial kits) were compared for their efficiencies in yield and quality of DNA obtained from four different tissues of four S. edule types. Clear effects of extraction methods, types and tissues of S. edule were observed on DNA yield and quality.

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