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ARTICLE

Endophytic fungi from Camellia sinensis show an antimicrobial activity against the rice blast pathogen Magnaporthe grisea

by Zhu XJ, YF Hu, X Chen, YH Wang, WP Fang, XH Li

Tea Science Research Institute, Nanjing Agricultural University, Nanjing 210095, P.R. China.
† These authors contributed equally to this work.

* Corresponding Author:Address Correspondence to: Xinghui Li, e-mail:

Phyton-International Journal of Experimental Botany 2014, 83(all), 57-63. https://doi.org/10.32604/phyton.2014.83.057

Abstract

The purpose of this study was to evaluate the antagonistic activity of two endophytic fungal strains, Pseudocercospora kaki and Penicillium sclerotiorum, isolated from the leaves of Camellia sinensis, against the rice blast pathogen Magnaporthe grisea. The inhibitory activity of the two endophytes against M. grisea in dual-culture was compared with that in monoculture. It was confirmed that the broth and its ethyl acetate extract of the dual-culture had a much stronger inhibition activity against M. grisea than the monocultures of P. kaki and P. sclerotiorum. The antagonism index of the broth and ethyl acetate extract from dual-culture to the mycelial growth of M. grisea was 78.02% ± 2.19% and 62.81% ± 2.29%, respectively, in different incubation periods. Qualitative analysis of ethyl acetate extract by GC-MS revealed that the number of bioactive compounds was greater in dual-culture than in monoculture. Compared to the ethyl acetate extract from monoculture, there were 10 constituents of bioactive compounds from dual-culture; however, there were only 6 types and 5 types from P. kaki monoculture and P. sclerotiorum monoculture, respectively. Glycerol; 4-Hydroxyphenyl ethanol; 1,2,3,4-Tetrahydroxy valeraldehyde and 1,2-Benzene-dicarboxylic acid dicyclohexyl ester were found in the ethyl acetate extracts of P. kaki, P. sclerotiorum and the dual culture. Based on the composition of the ethyl acetate extract of the dual culture of the endophytic fungi it might be possible to make a bioformulation for the biocontrol of the plant pathogen.

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