Open Access

ARTICLE

Evaluation in vitro of the anti-fungal activity of four methanol plant extracts for the control of three species of Fusarium spp.

Ochoa Fuentes YM¹, E Cerna Chávez¹, J Landeros Flores¹, S Hernández Camacho², JC Delgado Ortiz¹

1 Universidad Autónoma Agraria Antonio Narro, Departamento de Parasitología, Buenavista, Saltillo, Coahuila, C.P. 25315, México.
2 Universidad Autónoma de Aguascalientes, Centro de Ciencias Agropecuarias, Departamento de Fitotecnia, Posta zootécnica, Jesús María, Aguascalientes, C.P. 20900, México.

* Corresponding Authors:Address Correspondence to: Y isa María Ochoa Fuentes. Phone (01) 449 9650062 Ext 8107, e-mail: ;

Phyton-International Journal of Experimental Botany 2012, 81(all), 69-73. https://doi.org/10.32604/phyton.2012.81.069

Abstract

We evaluated the in vitro antifungal activity of extracts of peruvian peppertree (Shinu smolle), cherimoya (Annona cherimola), cinnamon (Cinnamomum zeylanicum) and tobacco (Nicotiana glauca) on the mycelial growth and sporulation of Fusarium oxysporum, Fusarium culmorum and Fusarium solani. The research was conducted at the Universidad Autónoma de Aguascalientes (UAA) and the Universidad Autónoma Agraria Antonio Narro (UAAAN). The species of Fusarium were previously identified in the UAA Laboratory of Parasitology. The extracts were obtained in the laboratory of Toxicology of the UAAAN. The methodology of poisoned culture medium was used to determine the (1) inhibition of mycelial growth, (2) average effective dose (ED₅₀) and (3) number of conidia at various concentrations of the extracts. The cherimoya and cinnamon extracts exhibited inhibitory effects on mycelial growth and sporulation. In contrast, tobacco and Peruvian peppertree extracts did not show any inhibitory effect. However, the first of them increased conidia production when extract concentration was increased. In relation to ED₅₀, cinnamon extracts were effective to control Fusarium species in doses of 330 to 539 ppm. On the other hand, the best control of the cherimoya extract was at 593 ppm against F. culmorum, while higher doses from 2060 to 2571 ppm were required to control F. oxysporum and F. solani, respectively. We conclude that cinnamon and cherimoya extracts showed inhibitory effects on the mycelial growth and sporulation of F. oxysporum, F. culmorum and F. solani.

---

Keywords

---