Open Access

ARTICLE

Obtaining new germplasm in Cenchrus ciliaris L. through induced-mutation and in vitro selection

by López Colomba E¹, A Prina², S Griffa¹, AN Ribotta¹, E Carloni¹, E Tommasino¹, C Luna¹, E Biderbost¹, K Grunberg¹

1 Área Resistencia al Estrés. Instituto de Fitopatología y Fisiología Vegetal - Instituto Nacional de Tecnología Agropecuaria (IFFIVE INTA). Camino 60 cuadras km 51/2. X5020I-CA. Córdoba. Argentina.
2 Instituto de Genética “Ewald A. Favret ”. Instituto Nacional de Tecnología Agropecuaria C.C. 25 (1712) Castelar. Buenos Aires. Argentina.

* Corresponding Author:Address Correspondence to: Dr. Eliana López Colomba, e-mail: ; Fax +54-351-4974330; Phone +54-351-4973636.

Phyton-International Journal of Experimental Botany 2011, 80(all), 59-64. https://doi.org/10.32604/phyton.2011.80.059

Abstract

Cenchrus ciliaris L., a forage grass of wide distribution in the north-west of Argentina, is a tetraploid (4x = 36) and obligate apomictic species. One way of obtaining novel germplasm is by induced mutations. In this work, physical and chemical mutations are combined with in vitro selection procedures seeking for new germplasm, with emphasis on salinity and drought tolerance. Mature seeds of Cenchrus ciliaris L. cv Biloela were subjected to treatments with X rays (400 Gy) and ethyl methanesulphonate (EMS) water solution (5.5 mM for 24 h). To perform in vitro selection, after 7 days of EMS or X rays treatments, germinated seeds were transferred to tubes containing Murashige and Skoog medium supplemented with NaCl or mannitol to simulate salinity and drought conditions, respectively. Fifty-four selected plants were isolated which tolerated 200 mM NaCl and 100 mM mannitol. Both mutagenic agents exhibited similar percentages of induced genetic variation measured through RAPD polymorphisms. This work demonstrated that genetic variability can be generated in Cenchrus ciliaris L. using mutagenic agents and in vitro selection.

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