Open Access

ARTICLE

AFLP marker application to explore heterosis in Capsicum spp.

by Castañón-Najera G¹, M Ramírez-Meraz³, R Ruiz-Salazar⁴, N Mayek-Pérez²

1 Profesor-Investigador. División Académica de Ciencias Biológicas (DACBiol), Universidad Juárez Autónoma de Tabasco (UJAT). Carretera Villahermosa-Cárdenas, Km 0.5 Entronque Bosques de Saloya, Villahermosa, Tab., México. C.P. 86000, Tel. / Fax 055-993-3-54-43-08.
2 Profesor-Investigador. Centro de Biotecnología Genómica (CBG), Instituto Politécnico Nacional (IPN). Boulevard del Maestro S/N, Col. Narciso Mendoza, Reynosa, Tam, México.
3 Investigador Titular. Campo Experimental Las Huastecas, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Villa Cuauhtémoc, Tampico, Tam.
4 Doctorando. Centro de Biotecnología Genómica (CBG), Instituto Politécnico Nacional (IPN). Boulevard del Maestro S/N, Col. Narciso Mendoza, Reynosa, Tam, México

* Corresponding Author:Address Correspondence to: Prof. Guillermo Castañón-Nájera, e-mail:

Phyton-International Journal of Experimental Botany 2011, 80(all), 53-58. https://doi.org/10.32604/phyton.2011.80.053

Abstract

The chili varieties: (1) Mirador, (2) Piquín huasteco, (3) Pico paloma, (4) Ozulamero and (5) Chilpaya Tabasco were crossed in a diallel without reciprocal crosses to produce 10 F1 hybrid combinations. While the previous varieties (1), (2) and (4) belong to Capsicum annuum varieties, (3) and (5) pertain to Caspicum frutescens. The four study AFLP markers amplified 282 bands or fragments, and of these, 264 were polymorphic. The estimated Genetic Parameters showed that there was dominance based on the genetic distances in the study DNA fragments. Heritability, which was estimated in a narrow sense, was moderate (0.53). This indicated the presence of dominant effects. Cluster analysis based on genetic distances separated parents and crosses of C. annuum from those of C. frutescens, confirming the genomic differences between the two species. Our results indicate that the Hayman methodology is appropriate to estimate heterosis and other important genetic parameters such as heritability. However, future research on Capsicum spp. should also (1) measure useful morphological characters, (2) estimate general and specific combining abilities, and (3) analyze simultaneously molecular and morphological information. These works would contribute to select the best parents in a breeding program for chili.

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