Open Access

ARTICLE

Cloning and expression analysis of rubredoxin from cold-treated banana leaves

Feng¹ RJ, LF Lu², KH Yuan³, P Cheng³, LL Zhang³, JF Qi³, Y Ren⁴, XL Xu⁵, XB Zhang³, LY Zhou³, YD Zhang³
1 Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou 571101, P.R. China.
2 Hainan Institute of Science &Technology, Haikou 571126, P.R. China.
3 College of Agronomy, Hainan University, Haikou 570228, P.R. China.
4 Tropical Crops Genetic Resources Institute, CATAS, Danzhou 571737, P.R. China.
5 The Environment and Plant Protection Institute, CATAS, Danzhou 571737, P.R. China.
* Corresponding Author:Address Correspondence to: YD Zhang, e-mail: ; fax 0086-898-66890978; Phone 0086-898-66279092.

Phyton-International Journal of Experimental Botany 2010, 79(all), 163-168. https://doi.org/10.32604/phyton.2010.79.163

Abstract

A banana (Musa AAA, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (MaRd1) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. MaRd1 cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of Pyrococcus furiosus rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of MaRd1 gene. Southern blotting revealed the occurrence of at least two copies of MaRd1 in the banana genome. Real time quantitative RT-PCR analysis revealed that the expression of MaRd1 gene was mainly in leaves, pseudo-stems and immature fruits, while it was barely detectable in roots and flowers. Cold and salt stresses induced higher levels of MaRd1 transcript accumulation in leaves. This finding indicated a role of MaRd1 in the response to these abiotic stresses.

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Keywords

Rubredoxin, Expression, Banana, Stress response.

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