Open Access

ARTICLE

Saturation of a durum wheat genetic map and detection of QTL associated to lipoxygenase activity

by Picca ¹, A, P Roncallo¹, A Carrera¹, G Cervigni¹, R. Miranda², V Echenique¹

1 CERZOS (CONICET) y Departamento de Agronomía, Universidad Nacional del Sur, San Andrés 800, 8000 Bahía Blanca, Argentina.
2 Criadero de Cereales de Invierno, Asociación de Cooperativas Argentinas y Departamento de Agronomía, Universidad Nacional del Sur, San Andrés 800, 8000 Bahía Blanca, Argentina.

* Corresponding Author:Address Correspondence to: Dr. Viviana Echenique, e-mail: ; fax 054-0291-4861124; Phone 054-0291-4861666.

Phyton-International Journal of Experimental Botany 2008, 77(all), 175-188. https://doi.org/10.32604/phyton.2008.77.175

Abstract

The aims of this work were (1) the saturation of a linkage map of durum wheat using AFPLs and RAPDs, (2) mapping of QTL related to lipoxygenase activity and (3) estimation of its usefulness in marker-assisted selection to increase pasta colour. A mapping population of 83 recombinant lines (RILs) derived from the cross between durum wheat Kofa and UC1113 (Triticum turgidum L. var. durum) was evaluated for lipoxygenase activity during two growing seasons. It was used to generate a genetic map. Forty four AFLPs, 9 RAPDs, 2 isoenzymes and 1 storage protein were mapped onto a previous genetic map consisting on 269 markers. The total length of the map obtained was 1847.4 cM, with an average genetic distance between pairs of markers of 5.68 cM. The composite interval mapping (CIM) showed the presence of a major QTL explaining 58% of phenotypic variation in lipoxygenase activity on chromosome 4B. The highest LOD value (LOD=19,00) was obtained between the “ksm62” and “wmc617b” markers. These results were consistent in the two sampling years and at the two pH in which lipoxygenase activity was analyzed.

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