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Classification and estimation of genetic diversity in Nopal Opuntia spp based on phenotypic descriptors and genetic-molecular markers

García-Zambrano2 Eduardo A, Adriana Gutiérrez-Díez, Gilberto E Salinas-García, Elizabeth Cárdenas-Cerda, Rigoberto E Vázquez-Alvarado, Francisco Zavala-García, Jesús Martínez de la Cerda
Trabajo realizado en la Facultad de Agronomía , Universidad Autónoma de Nuevo León. Apartado Postal 358, San Nicolás de los Garza N.L. C.P. 66450. México
Maestro-Investigador Facultad de Agronomía, Universidad Autónoma de Nuevo León, México eagarci1@hotmail.com.

Phyton-International Journal of Experimental Botany 2006, 75(all), 125-135. https://doi.org/10.32604/phyton.2006.75.125

Abstract

The objective of this investigation was to develop and apply molecular markers (type RAPD) for estimating the genetic diversity of 100 Opuntia accessions from the Germoplasma Bank of the FAUANL. The molecular data were evaluated by multivariate analysis using the methods of Ward and UPGMA. These results were compared with phenotypic data taken from the same accessions, already evaluated by the above mentioned methods. The protocol was modified for the extraction of the Opuntia DNA to reduce the quantity of polysaccharides and their influence in its quality. Later on, a protocol was developed for the generation of molecular markers of the type RAPD for Opuntia accessions. This allowed to combine the accessions by the similarity degree of the molecular markers, in agreement with phenotype data.

Keywords

molecular markers, RAPD, accesion, PCR

Cite This Article

, G., Salinas-García, G. E., Cárdenas-Cerda, E., Vázquez-Alvarado, R. E., Zavala-García, F. et al. (2006). Classification and estimation of genetic diversity in Nopal Opuntia spp based on phenotypic descriptors and genetic-molecular markers. Phyton-International Journal of Experimental Botany, 75(all), 125–135.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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