Open Access

ARTICLE

In vitro multiplication of the Piñón Azul Pinus maximartinezii (Rzedowski)

Ojeda-Zacarías¹ Ma del Carmen, Hugo A Luna-Olvera², Lilia H Morales-Ramos², María J Verde-Star², Teresa E Torres-Cepeda², Benito Pereyra-Alférez², Leobardo Iracheta-Donjuan³, Emilio Olivares-Sáenz⁴, Raúl Salazar-Sáenz⁴, Elizabeth Cárdenas-Cerda⁴
1 Instituto Tecnológico de Nuevo León, Eloy Cavazos No 2001, Guadalupe, Nuevo León, CP 67170.
2 Instituto de Biotecnología, Facultad de Ciencias Biológicas, Apdo Postal 38F, Pedro de Alba, s/n, Cd. Universitaria, San Nicolás de los Garza, N.L. México, CP. 66450.
3 Investigador del Instituto Nacional Investigaciones Forestales, Agrícolas y Pecuarias; Campo Experimental Rosario Izapa, Tuxtla Chico Chiapas, Méx. CP. 30870
4 Laboratorio de Biotecnología Vegetal, Facultad de Agronomía, Carretera Zuazua-Marín Km 17.5, Marín, N.L., CP. 66700, e-mail ojedacz@yahoo.com.mx
Agradecimiento: al Consejo Nac. De Ciencia y Tecnología por el apoyo económico brindado en la realización de mis estudios Doctorales.

Phyton-International Journal of Experimental Botany 2006, 75(all), 109-113. https://doi.org/10.32604/phyton.2006.75.109

Abstract

Pinus maximartinezii (Rzedowski) is an endemic endangered species, confined to a single population of approximately 2000 to 2500 mature trees. It covers about 400 ha in southern Zacatecas, Mexico. The success of tissue culture techniques for germplasm preservation depends on regeneration of cultures. The objective of this study was to achieve an in vitro proliferation protocol using organogenesis technique for Pinus maximartinezii. Mature seeds were surface sterilized in 6% H2O2 v/v. Isolated cotyledons and zygotic embryos were cultured on shoot induction media. DCR and GD media were supplemented with 0.3 and 0.5 mgl-1 BAP; 0.01 mgl-1 ANA and vitamin solution. Explants were incubated at 26 ± 2ºC under a 16h photoperiod. The explants were transferred every 15 days to hormone-free medium (DCR and GD) for a period of 6 wk for bud development. The number of explants forming buds was determined. After induction of buds, the explants were transferred to a hormone-free basal medium, to which 0.1% activated charcoal was added. After 8 wk, the number of shoots per embryo was evaluated. Effects of either basal media or plant growth regulator concentrations were significantly different (p<0.05).

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Keywords

Micropropagation, conifers, Piñon Azul, foliar primordial, organogénesis

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