Open Access

ARTICLE

Concentration analysis in steay-state of ascorbate radical in soybean seedlings determined by electronic paramagnetic resonancy

by Galatro Andrea, Ivan Rousseau, Susana Puntarulo

Fisicoquímica-PRALIB, Departamento de Química Analítica y Fisicoquímica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
Correspondencia a: Dra. Susana Puntarulo -Fisicoquímica-PRALIB, Facultad de Farmacia y Bioquímica, Junín 956 (C1113AAD) Buenos Aires, Argentina, Phone: 54114964-8244 Ext: 101 / Fax: 5411-4508-3646 Ext: 102 - e-mail: susanap@ffyb.uba.ar
Este trabajo ha sido financiado por subsidios provenientes de la Universidad de Buenos Aires, el CONICET y la Agencia Nacional de Promoción Científica y Técnica. SP es miembro de la carrera del CONICET, AG se desempeña como becaria post-doctoral del CONICET e IR es becario de la Agencia Nacional de Promoción Científica y Técnica.

Phyton-International Journal of Experimental Botany 2006, 75(all), 7-20. https://doi.org/10.32604/phyton.2006.75.007

Abstract

Electron Paramagnetic Resonance (EPR) has been developed as a versatile field that uses different techniques sharing the common feature of resonant micro-wave radiation absorption by paramagnetic substances. Certain free radicals, such as ascorbyl radical (A•), show an stable spectrum, and can be directly detected by EPR at room temperature. Studies included in this work clearly show that this highly sensitive methodology can be successfully applied to biological systems. Exposure of plants to UV-B radiation was studied as a factor of oxidative stress in plants, and the effect on A^• steady state concentration was analyzed. A kinetic analysis was performed considering a simple physical chemistry mechanism, which allowed the estimation of A^• steady state concentration that properly parallels experimental values obtained by EPR.

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