Guest Editors
Jinhui Chen, Professor, Nanjing Forestry University, Nanjing 210037, China
Xiangyang Hu, Professor, Shanghai University, Shanghai, 200444, China
Summary
Somatic embryogenesis (SEs) technique is one of important tool to study plant embryo development and regulation, as well as the industrial breeding of rare and endogenous tree species, thus shows essential for fundamental research or production application. it allows the long-term ex situ conservation of genetic resources by cryopreservation techniques. For some woody species and in particular for some coniferous trees, somatic embryogenesis induction has become a routine procedure. For the majority, the application of this technology presents yet many limitations especially due to the genotype, the induction conditions, the number of embryos produced, maturation, and conversion, among other factors that compromise the systematic use of somatic embryogenesis for commercial purposes especially of woody species and forest trees in particular. In recent years there has been major improvement in tree SEs, this special issue will describe progress in SEs of tree SEs and its application in tree breeding and application.
This special issue is proposed to explore the most recent development and innovation occurs during recent years in the field of tree somatic embryogenesis and application. The manuscripts address the following topics:
1) the novel fundamental of tree somatic embryogenesis
2) the key regulatory factor to determine tree somatic embryogenesis
3) the omics study of tree somatic embryogenesis
3) the tree tissue culture and regeneration
4) the new or high-efficiency technique for transgenic tree
5) the genome editing for tree
6) the industrial application of tree somatic embryogenesis
Keywords
Tree, Somatic Embryogenesis, Industrial Application
Published Papers
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Open Access
ARTICLE
Vital Parameters Assessments of Starvation Tolerance of in vitro Populus alba Culture
Mohamed Fathy Ahmed, Eman Zakaria Ahmed, Salman Aloufi, Mohammed Alqurashi, Amal Alyamani, Eman Fayad, Eman Tawfik Hussien
Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 889-902, 2022, DOI:10.32604/phyton.2022.018600
（This article belongs to this Special Issue:
Tree Somatic Embryogenesis and Application)
Abstract Populus alba is a large woody deciduous plant. The plant has been introduced to shooting, then multiplication of rooting on Murashige and Skoog (MS) medium. This work was designed to estimate the effect of two factors (low levels of 1-Naphthaleneacetic acid NAA and sucrose) on
P. alba response resulting in 6 treatments compared to the control, with twelve measured responses. There was a significant difference in some measurements in morphology, like plantlets fresh-weight, shoot-, root-length, and leaf number. In the physiological measurements, there were significant differences in all the measured parameters. The low concentrations of sucrose and media composition/power (MS…
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Open Access
ARTICLE
Suspension Culture and Somatic Embryogenesis of Korean Pine
Chunxue Peng, Fang Gao, Hao Wang, Iraida Nikolaevna Tretyakova, Alexander Mikhaylovich Nosov, Hailong Shen, Ling Yang
Phyton-International Journal of Experimental Botany, Vol.91, No.1, pp. 223-238, 2022, DOI:10.32604/phyton.2022.015523
（This article belongs to this Special Issue:
Tree Somatic Embryogenesis and Application)
Abstract Korean pine is an important afforestation tree species in Northeast China, which has a high ecological and economic value. Although regeneration of somatic embryogenesis using immature zygotic embryos of Korean pine as explants has been successful, it cannot be applied to automation and large-scale production. Therefore, we urgently need a method that can increase the output of somatic embryos (SEs) to meet the needs of large-scale production. We used Korean pine 1–1 and 1–100 cell lines as research materials to evaluate the effects of inoculum-density, culture time, orbiting speed, vessel volume, plant growth regulator (PGR) concentration, and carbon source on…
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Open Access
ARTICLE
Encapsulation of Immature Somatic Embryos of Coffea arabica L. for in Vitro Preservation
Eliana Arias-Pérez, Carlos Alberto Lecona-Guzmán, Federico Antonio Gutiérrez-Miceli, Joaquín Adolfo Montes-Molina, Nancy Ruiz-Lau
Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1741-1748, 2021, DOI:10.32604/phyton.2021.016004
（This article belongs to this Special Issue:
Tree Somatic Embryogenesis and Application)
Abstract The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos (
Coffea arabica L.), for the conservation of genotypes with characteristics of commercial interest. Somatic embryos were induced from leaf explants in Murashige and Skoog medium (MS) supplemented with 1 mg · L
−1 of 2,4-dichlorophenoxiacetic acid (2,4-D) combined with 2 mg · L
−1 of benzyladenine (BA). Somatic embryos (SE) at the globular stage were encapsulated in a sodium alginate matrix; two treatments were tested: MS + 5 mg · L
−1 BA + 1 mg · L
−1 NAA + 3% (w/v) alginate, and MS + 7 mg · L
−1 BA + 5.7 mg · L
−1 indoleacetic acid (IAA) + 3% (w/v) alginate. Alginate was…
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Open Access
ARTICLE
The Regulatory Roles of microRNAs and Associated Target Genes during Early Somatic Embryogenesis in Liriodendron Sino-Americanum
Dan Wang, Fengjuan Lu, Pengkai Wang, Jiaji Zhang, Lu Lu, Yan Zhen, Ye Lu, Tielong Cheng, Jisen Shi, Jinhui Chen
Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1445-1463, 2021, DOI:10.32604/phyton.2021.015499
（This article belongs to this Special Issue:
Tree Somatic Embryogenesis and Application)
Abstract Somatic cells respond to considerable stress, and go through a series of phytohormone pathways, then forming an embryo. The developmental process is recorded as somatic embryogenesis (SE). One of the key components regulating SE are the microRNAs (miRNAs). Despite previous studies, it is still not clear exactly how miRNAs exert their function of regulating targets during conditionally activated early SE. Here, we use
Liriodendron sino-americanum as a model system and perform a combined analysis of microfluidic chips and degradome sequencing to study this process. We identified a total of 386 conserved miRNAs and 153 novel miRNAs during early SE. According…
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Open Access
ARTICLE
Efficient Evergreen Plant Regeneration of Cinnamomum japonicum Sieb. through in vitro Organogenesis
Jiaji Zhang, Xiaofei Long, Yuhao Weng, Tielong Cheng, Jisen Shi, Jinhui Chen
Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 571-582, 2021, DOI:10.32604/phyton.2021.014191
（This article belongs to this Special Issue:
Tree Somatic Embryogenesis and Application)
Abstract Cinnamomum japonicum Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study,
we successfully developed a large-scale micropropagation protocol for
C. japonicum for the first time. Sterilized shoots were excised and used as explants for
shoot induction on several basal media, supplemented with different concentrations
of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N
6
-Benzyladenine
(6-benzylaminopurine) (BA),
α-naphthaleneacetic acid (NAA) and Gibberellic acid
(GA
3). After comparison, the most efficient medium for shoot regeneration
was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L
–1 BA,
0.05 mg L
–1 NAA and…
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Open Access
ARTICLE
Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch
Kuipeng Li, Hua Han, Yunhui Xie, Xiaomei Sun
Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 583-593, 2021, DOI:10.32604/phyton.2021.013912
（This article belongs to this Special Issue:
Tree Somatic Embryogenesis and Application)
Abstract Optimization of
in vitro adventitious root induction contributes to the development of a large-scale production
system of hybrid clone seedlings of larch (
Larix spp.). We used orthogonal testing to investigate the factors that
affect the rooting of hybrid larch shoots–activated carbon, vitamin B1, sucrose, glycine, glutamic acid (Glu),
cysteine, pH, and induction time in the dark and indole-3-butyric acid (IBA). Variance analysis showed that
the effects of sucrose concentration on rooting rate, survival rate, and average root number, pH on the average
number of roots; and Glu on survival rate were all significant (
p < 0.05) when compared to the…
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Open Access
ARTICLE
An Efficient Plant Regeneration System of Hydrangea bretschneideri Dipp via Stem Segments as Explants
Si ran Wang, Jia Yan, Bu er Ha, Yu’e Bai
Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 595-604, 2021, DOI:10.32604/phyton.2021.013693
（This article belongs to this Special Issue:
Tree Somatic Embryogenesis and Application)
Abstract Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration. Genetic engineering
technology has been successfully used in many plant species, but it is limited in
Hydrangea. Here we established
an efficient regeneration system by using stem segments as explants for the first time. In our study, the plant
growth regulators (PGRs) were evaluated at the different regeneration processes, including axillary shoots regeneration and root induction. We found that the optimal concentration for axillary buds’ induction was 2.0 mgL
−1
6-BA and 0.5 mgL
−1
1 IAA, its highest induction rate was 70%. Moreover, the highest axillary shoots proliferation
coefficient…
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