Optimization of Agrobacterium tumefaciens-Mediated Genetic Transformation of Maize
Mengtong Liu, Yangyang Zhou, Tongyu Liu, Jianyu Lu, Jing Qu, Shuyan Guan, Yiyong Ma
Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 363-374, 2022, DOI:10.32604/phyton.2022.016325
(This article belongs to the Special Issue:
Plant Physiology for Crop Production and Sustainable Agriculture)
Abstract Immature embryos of inbred maize (
Zea mays) lines (H8183, H8184, and H8185) were used for
Agrobacterium infection. We used the β-glucuronidase gene (
GUS) as the target gene and the glufosinate resistance gene (
bar) as the selection marker. We conducted research on several aspects, such as different genotypes, coculture conditions, screening agent concentrations, and concentrations of indole-3-butytric acid (IBA), 6-benzylaminopurine (6-BA), and ascorbic acid (Vc) in the differentiation medium. We optimized the genetic transformation system, and the obtained results indicated that among the three lines studied, the induction rate of H8185 was the highest at 93.2%, followed by H8184,
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