OsMAPK6 Affects Male Fertility by Reducing Microspore Number and Delaying Tapetum Degradation in Oryza sativa L.
Wuzhong Yin, Hongxia Yang, Yantong Wang, Ping Feng, Yao Deng, Yang Liu, Danyang Chen, Yijie Ban, Weichi Liu, Guanghua He, Nan Wang
Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 727-744, 2022, DOI:10.32604/phyton.2022.017811
(This article belongs to the Special Issue:
High-Yield Rice Physiology & Genetics)
Abstract The mitogen-activated protein kinase (MAPK) cascade is important in stress signal transduction and plant development. In the present study, we identified a rice (
Oryza sativa L.) mutant with reduced fertility,
Oryza sativa
mitogen-activated protein kinase 6 (osmapk6), which harbored a mutated MAPK gene. Scanning and transmission
electron microscopy, quantitative RT-PCR analysis, TUNEL assays, RNA in situ hybridization, longitudinal and
transverse histological sectioning, and map-based cloning were performed to characterize the
osmapk6 mutant.
The gene
OsMAPK6 was expressed throughout the plant but predominantly in the microspore mother cells, tapetal cells, and microspores in the anther sac. Compared with the
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