Genome-Wide Identification of the MYB Gene Family and Screening of Potential Genes Involved in Fatty Acid Biosynthesis in Walnut
Dongxue Su1, Jiarui Zheng1, Yuwei Yi1, Shuyuan Zhang1, Luxin Feng1, Danzeng Quzhen2, De Qiong3, Weiwei Zhang1, Qijian Wang1, Feng Xu1,*
1 College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
2 Lhasa Municipal Forestry and Grassland Bureau, Lhasa, 850000, China
3 Agro-Tech Extension and Service Center, Shannan, 856000, China
* Corresponding Author: Feng Xu. Email: 
Phyton-International Journal of Experimental Botany https://doi.org/10.32604/phyton.2024.055350
Received 24 July 2024; Accepted 19 August 2024; Published online 20 September 2024
Abstract
The multifaceted roles of
MYB transcriptional regulators are pivotal in orchestrating the complex processes of secondary metabolism, stress tolerance mechanisms, and life cycle progression and development. This study extensively examined the
JrMYB genes using whole genome and transcriptomic data, focusing on identifying putative
MYB genes associated with fatty acid metabolism. 126
MYB genes were identified within the walnut genome, characterized by hydrophilic proteins spanning lengths ranging from 78 to 1890 base pairs. Analysis of cis-acting elements within the promoter regions of
MYB genes revealed many elements linked to cell development, environmental stress, and phytohormones. Transcriptomic data was utilized to examine the role of
JrMYB genes in the biosynthesis of fatty acids in walnuts. The results revealed diverse expression of these genes across various tissue sites, displaying varying levels and distinct expression patterns. Furthermore, by integrating the results of the phylogenetic tree with the correlation of expression levels, a total of 10 genes potentially involved in the regulation of fatty acid synthesis were screened. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was conducted on these 10 genes and further identified 4 candidate genes, and a transcription regulatory network involved in fatty acids metabolism was constructed. This study presents a systematic analysis of
JrMYB genes, laying the groundwork for an in-depth exploration of the
JrMYB genes family’s function in regulating fatty acid synthesis.
Graphical Abstract
Keywords
Walnut;
JrMYB genes; fatty acid synthesis; gene expression; bioinformatics analysis
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