Open Access
ARTICLE
Chemically Mediated Interactions between Grapevine, Aphid, Ladybird, and Ant in the Context of Insect Chemical Ecology
Taghreed Alsufyani1,*, Noura J. Alotaibi2, Nour Houda M’sakni1, Mona A. Almalki1, Eman M. Alghamdi3
1 Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia
2 Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia
3 Chemistry Department, Faculty of Science, King AbdulAziz University, P.O. Box 80200, Jeddah, 21589, Saudi Arabia
* Corresponding Author: Taghreed Alsufyani. Email:
Phyton-International Journal of Experimental Botany https://doi.org/10.32604/phyton.2024.050351
Received 04 February 2024; Accepted 24 May 2024; Published online 10 July 2024
Abstract
This study simplifies the complex relationship among grapevine plants, aphids, ladybirds, and ants, which is
essential for effective pest management and ecological balance. This study investigated the impact of aphid
attacks and the presence of ants and ladybirds on the volatile compounds profile released into the chemosphere
of the community consisting of the common vine
Vitis vinifera, the aphid
Aphis illinoisensis, the ladybird
Coccinella undecimpunctata-and the ant
Tapinoma magnum. This study aims to analyze the volatile compounds
emitted by the grapevine and surrounding insects in response to these intricate interactions. The extraction of
volatile organic compounds (VOCs) was carried out using closed-loop stripping (CLS) and then analyzed via
gas chromatography-mass spectrometry (GC-MS) and principles coordinated analysis (PCA) was performed.
The grapevine was exposed to different types and order of treatments, including non-infested, aphid-infested,
aphid-infested with ant, aphid-infested with ladybird, and various combinations of ant and ladybird. After the
aphid attack, the outcomes uncovered massive alterations in the volatile compound profiles. Infested grapevine
displayed distinct emissions of germacrene D, an alcohol, and an alkene compared to non-infested plants. The
characteristic VOC profile was the share of infested grapes in the presence of ants, with benzene derivatives and
sesquiterpenes dominating the components. The coexistence of ladybirds with ants and aphids resulted in a different volatile profile characterized by elevated levels of aldehydes, ketones, α-farnesene, and its hydroxy derivative. It was concluded that the emission of VOCs into the chemosphere of the grapevine communities varied
qualitatively and quantitatively depending on the level of the relationship complexity within each community
in response to the infestation of grapevines by aphids, the presence of ladybirds as natural predators, and the
presence of ant as protector. The grapevine’s status-dependent compounds can serve as indicators of infestation
status and contribute to non-destructive early-stage diagnosis of the aphid.
Keywords
Aphids;
Aphis illinoisensis; volatile organic compounds; chemosphere; pest management; gas chromatography-mass spectrometry; closed-loop stripping