Open Access
ARTICLE
GhSCL4 Acts as a Positive Regulator in Both Transgenic Arabidopsis and Cotton during Salt Stress
Yanyan Zhao1,*, Yanpeng Ding2, Bailin Duan1, Qingzhou Xie1
1
Plant Genomics & Molecular Improvement of Colored Fiber Lab, College of Life Sciences and Medicine, Zhejiang Sci-Tech
University, Hangzhou, 310018, China
2
China Agricultural University, Beijing, 100193, China
* Corresponding Author: Yanyan Zhao. Email:
(This article belongs to this Special Issue: Symbiotic Associations for Nutrients Management and Complexes Formation for Better Agricultural Crops Productivity under Biotic and Abiotic Stresses)
Phyton-International Journal of Experimental Botany 2023, 92(1), 1-15. https://doi.org/10.32604/phyton.2022.022384
Received 08 March 2022; Accepted 13 April 2022; Issue published 06 September 2022
Abstract
GRAS transcription factors play important roles in plant abiotic stress response, but their characteristics and
functions in cotton have not been fully investigated. A cotton SCL4/7 subgroup gene in the GRAS family,
GhSCL4, was found to be induced by NaCl treatments. Nuclear localization and transactivation activity of
GhSCL4
indicate its potential role in transcriptional regulation. Transgenic Arabidopsis thaliana over-expressing
GhSCL4
showed enhanced resistance to salt and osmotic stress. What’s more, the transcript levels of salt stress-induced
genes (AtNHX1 and AtSOS1) and oxidation-related genes (AtAPX3 and AtCSD2) were more highly induced in
the
GhSCL4 over-expression lines than in wild type after salt treatment. Furthermore, silencing of
GhSCL4
resulted in reduced salt tolerance in cotton caused by reactive oxygen species (ROS) enrichment under salt treatment, and antioxidant enzyme activities were accordingly significantly reduced in GhSLC4-silenced lines. These
results indicated that
GhSCL4 enhances salt tolerance of cotton by detoxifying ROS. In addition, the transient
expression assay confirmed an interactive relationship between
GhSCL4 and GhCaM7, which indicated that salt
tolerance conferred by
GhSCL4 might be associated with salt-induced Ca
2+/CaM7-mediated signaling. Taken
together,
GhSCL4 acts as a positive regulator in cotton during salt stress that is potentially useful for engineering
salt-tolerant cotton.
Keywords
Supplementary Material
Supplementary Material File
Cite This Article
Zhao, Y., Ding, Y., Duan, B., Xie, Q. (2023).
GhSCL4 Acts as a Positive Regulator in Both Transgenic
Arabidopsis and Cotton During Salt Stress.
Phyton-International Journal of Experimental Botany, 92(1), 1–15.