Open Access

ARTICLE

In Vitro Propagation of Agave guiengola Gentry Using Semisolid Medium and Temporary Immersion Bioreactors

by Lucía Isabel Chávez-Ortiz¹, José Francisco Morales-Domínguez¹, Araceli Rodríguez-Sahagún², Eugenio Pérez-Molphe-Balch^1,*

1 Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, 20131, México
2 Laboratorio de Biología Molecular Vegetal, Centro Universitario de la Ciénega, Universidad de Guadalajara, Ocotlán, 47820, México

* Corresponding Author: Eugenio Pérez-Molphe-Balch. Email:

Phyton-International Journal of Experimental Botany 2021, 90(3), 1003-1013. https://doi.org/10.32604/phyton.2021.012862

Received 15 July 2020; Accepted 09 October 2020; Issue published 30 March 2021

Abstract

Agave guiengolaGentry is an endemic plant from a very small locality in Oaxaca, Mexico. Its conservation status is fragile and can rapidly worsen. Because of its scarcity, this agave has been used solely for ornamental purposes, but it could have other uses if more plants were available. In vitro propagation by enhanced axillary sprouting from stem segments was attained using Murashige and Skoog Basal Medium (MS) as well as basal medium supplemented with cytokinins 6-Benzylaminopurine (BA) or 6-(γ,γ-Dimethylallylamino)purine (2iP). The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l^–1 BA, obtaining a mean of 3.7 shoots per explant. Other interesting responses were observed, such as nodular callus induction using combinations of BA and 2,4-Dichlorophenoxyacetic acid (2,4-D); root induction without Plant Growth Regulators (PGR); and generation of shoot clusters. These clusters constituted an excellent explant for micropropagation in temporary immersion bioreactors, obtaining a propagation rate of 43 shoots per explant with 1 min immersion and 6 h immersion frequencies. All new plants rooted and survived the transfer to soil. This study developed an in vitro propagation scheme to produce individuals that can be used either for reforestation, economical purposes, or to carry out studies in this species to assess its full potential, avoiding exploitation from wild plants.

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