Open Access
ARTICLE
Effects of Exogenous Manganese (Mn) on Mineral Elements, Polyamines and Antioxidants in Apple Rootstock Malus robusta Rehd.
Dazhuang Qi1,2,4,#, Meixia Liang1,2,4,#, Fudong Jiang3, Jianzhao Li1,2,4, Xuqiang Qiao1,2,4,*, Hongxia Zhang1,2,4,*
1 College of Agriculture, Ludong University, Yantai, 264025, China
2 Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, 264025, China
3 Yantai Academy of Agricultural Science, Yantai, 264025, China
4 Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong (Ludong University), Yantai, 264025, China
* Corresponding Authors: Xuqiang Qiao. Email: ; Hongxia Zhang. Email:
# These authors contribute equally to this work
Phyton-International Journal of Experimental Botany 2020, 89(4), 943-955. https://doi.org/10.32604/phyton.2020.013801
Received 20 August 2020; Accepted 01 September 2020; Issue published 09 November 2020
Abstract
Manganese (Mn) is one of the essential microelements in all organisms.
However, high level of Mn is deleterious to plants. In this study, the effects of exogenous manganese application on mineral element, polyamine (PA) and antioxidant
accumulation, as well as polyamine metabolic and antioxidant enzyme activities,
were investigated in
Malus robusta Rehd., a widely grown apple rootstock. High
level of Mn treatments decreased endogenous Mg, Na, K and Ca contents, but
increased Zn content, in a Mn-concentration-dependent manner. Polyamine metabolic assays revealed that, except the content of perchloric acid insoluble bound
(PIS-bound) spermine, which increased significantly, the contents of putrescine
(Put), spermidine (Spd) and spermine (Spm) all decreased progressively, accompanied with the decreased activities of arginine decarboxylase (ADC, EC 4.1.1.19)
and ornithine decarboxylase (ODC, EC 4.1.1.17), and the increased activities of
diamine oxidase (DAO, EC 1.4.3.6) and polyamine oxidase (PAO, EC 1.5.3.3).
Further antioxidant capacity analyses demonstrated that contents of anthocyanin,
non-protein thiols (NPT) and soluble sugar, and the activities of guaiacol peroxidase
(POD, EC 1.11.1.7), catalase (CAT, EC 1.11.1.6) and superoxide dismutase (SOD,
EC 1.15.1.1), also increased upon different concentrations of Mn treatments. Our
results suggest that endogenous ion homeostasis is affected by high level of Mn
application, and polyamine and antioxidant metabolism is involved in the responses
of
M. robusta Rehd. plants to high level of Mn stress.
Keywords
Cite This Article
Qi, D., Liang, M., Jiang, F., Li, J., Qiao, X. et al. (2020). Effects of Exogenous Manganese (Mn) on Mineral Elements, Polyamines and Antioxidants in Apple Rootstock
Malus robusta Rehd..
Phyton-International Journal of Experimental Botany, 89(4), 943–955. https://doi.org/10.32604/phyton.2020.013801