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  • Open Access

    ARTICLE

    Cloning, Characterization and Transformation of Methyltransferase 2a Gene (Zmet2a) in Maize (Zea mays L.)

    Xin Qi1,#, Yu Wang1,#, Xing Zhang1, Xiaoshuang Wei1, Xinyang Liu1, Zhennan Wang1, Zhenhui Wang1,*, Fenglou Ling2,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.052844
    Abstract DNA methylation is an important epigenetic regulatory mechanism, it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s) to DNA. In this study, a novel methyltransferase 2a gene (Zmet2a) was cloned in maize and identified by polymerase chain reaction-base (PCR-base) using a bioinformatics strategy. The Zmet2a cDNA sequence is 2739 bp long and translates to 912 amino acid peptides. The Zmet2a protein revealed that it contains BAH and CHROMO structural domains, is a non-transmembrane protein that is hydrophilically unstable, and has no signal peptide structure. Meanwhile, we verified More >

  • Open Access

    ARTICLE

    Genetic Variability and Phenotypic Correlations Study among Grain Quality Traits and Mineral Elements Concentrations in Colored and Non-Colored Rice (Oryza sativa L.)

    Adel A. Rezk1,2,*, Mohamed M. El-Malky3, Heba I. Mohamed4,*, Hossam S. El-Beltagi1,5
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.052739
    (This article belongs to the Special Issue: Recent Research Trends in Genetics, Genomics, and Physiology of Crop Plants)
    Abstract Twenty-four rice genotypes were examined to assess genetic variability, heritability, and correlations for sevengrain quality traits, eight nutritional elements, and protein. ANOVA revealed significant differences for the quality traits studied. For every trait under study, the phenotypic coefficient of variation was higher than the correspondence genotypic coefficient of variation. Heritability in a broad sense varied from 29.75% for grain length to 98.31% for the elongation trait. Hulling percentage recovery had a highly significant positive correlation with milling and head rice percentage. Consequently, milling percentage had a highly positive correlation with head rice percentage. In amylose… More >

  • Open Access

    ARTICLE

    Combined Application of Biostimulants and EDTA Improved Wheat Productivity under Cadmium Stress

    Abida Aziz1, Shafiqa Bano1, Mubshar Hussain2, Muhammad Farooq Azhar3, Ghulam Yasin3, Naila Hadayat4, Iqra Arooj5, Abeer Hashem6, Ajay Kumar7, Elsayed Fathi Abd_Allah8, Qamar uz Zaman9,10,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.050974
    (This article belongs to the Special Issue: Abiotic Stress Impacts on Plant Physiology and Their Alleviation)
    Abstract Wheat (Triticum aestivum L.) exhibits a greater capacity for cadmium (Cd) absorption compared to other cereal crops, leading to elevated daily Cd intake, and posing a significant threat to public health. For the mitigation of Cd stress in sustainable and environmentally friendly way, a pot study was designed by using exogenous application of various biostimulants, i.e., Nigella sativa and Ocimum sanctum extracts: 0%, 10%, and 20% in combination with the chelating agent ethylenediaminetetraacetic acid (EDTA) using 0 and 5 mg kg under various levels of Cd stress (i.e., 0, 5, 10, and 15 mg kg soil). Results revealed… More >
    Graphic Abstract

    Combined Application of Biostimulants and EDTA Improved Wheat Productivity under Cadmium Stress

  • Open Access

    ARTICLE

    Selenium Regulates Antioxidant Capacities and Diterpenoid Biosynthesis in the Medicinal Plant Isodon rubescens

    Fenglan Zhao1,2,#, Shuwen Wu1,#, Xue Meng1, Jianping Xue1,2, Yongbo Duan1,2,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.052287
    Abstract Dōng líng căo, the dried aboveground parts of Isodon rubescens (Hemls.) Hara., is commonly consumed as a medicinal decoction or tea beverage. Natural beverages can be an important source of human dietary selenium (Se). However, how I. rubescens plants respond to exogenous Se remains unknown. In this study, a pot cultivation experiment was employed to investigate the phenotypic and physiological responses of I. rubescens plants exposed to Se. Fifteen days after applying different concentrations of sodium selenate to the soil, the Se enrichment capacity, growth indices, antioxidant capacities, and the content of flavonoids and diterpenoids were measured in… More >

  • Open Access

    ARTICLE

    Chemically Mediated Interactions between Grapevine, Aphid, Ladybird, and Ant in the Context of Insect Chemical Ecology

    Taghreed Alsufyani1,*, Noura J. Alotaibi2, Nour Houda M’sakni1, Mona A. Almalki1, Eman M. Alghamdi3
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.050351
    Abstract This study simplifies the complex relationship among grapevine plants, aphids, ladybirds, and ants, which is essential for effective pest management and ecological balance. This study investigated the impact of aphid attacks and the presence of ants and ladybirds on the volatile compounds profile released into the chemosphere of the community consisting of the common vine Vitis vinifera, the aphid Aphis illinoisensis, the ladybird Coccinella undecimpunctata-and the ant Tapinoma magnum. This study aims to analyze the volatile compounds emitted by the grapevine and surrounding insects in response to these intricate interactions. The extraction of volatile organic compounds (VOCs) was carried… More >

  • Open Access

    ARTICLE

    Comparative Analysis of the Essential Oil of the Underground Organs of Valeriana spp. from Different Countries

    Ain Raal1, Valeriia Kokitko2, Vira Odyntsova2, Anne Orav3, Oleh Koshovyi1,4,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.053754
    Abstract Valeriana officinalis L. is a plant from the Caprifoliaceae family, which is widely distributed in various parts of the world, especially in Europe and Asia. All species of Valeriana are distinguished by their ability to synthesize essential oil, which has a powerful effect on the physiological and mental aspects of the human body. The aim was to study the qualitative and quantitative composition of essential oil from valerian roots, collected in different countries, using the gas chromatography method, and to establish marker compounds for valerian species. 13 samples of commercial roots with rhizomes of V. officinalis from nine… More >

  • Open Access

    ARTICLE

    Genome-Wide Discovery and Expression Profiling of the SWEET Sugar Transporter Gene Family in Woodland Strawberry (Fragaria vesca) under Developmental and Stress Conditions: Structural and Evolutionary Analysis

    Shoukai Lin1,3,4,*, Yifan Xiong2, Shichang Xu1,2, Manegdebwaoaga Arthur Fabrice Kabore2, Fan Lin5, Fuxiang Qiu1,2,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.050990
    Abstract The SWEET (sugar will eventually be exported transporter) family proteins are a recently identified class of sugar transporters that are essential for various physiological processes. Although the functions of the SWEET proteins have been identified in a number of species, to date, there have been no reports of the functions of the SWEET genes in woodland strawberries (Fragaria vesca). In this study, we identified 15 genes that were highly homologous to the A. thaliana AtSWEET genes and designated them as FvSWEET1FvSWEET15. We then conducted a structural and evolutionary analysis of these 15 FvSWEET genes. The phylogenetic analysis enabled us… More >

  • Open Access

    REVIEW

    Artificial Intelligence for Maximizing Agricultural Input Use Efficiency: Exploring Nutrient, Water and Weed Management Strategies

    Sumit Sow1,#, Shivani Ranjan1,#,*, Mahmoud F. Seleiman2,3, Hiba M. Alkharabsheh4,*, Mukesh Kumar1, Navnit Kumar1, Smruti Ranjan Padhan5, Dhirendra Kumar Roy1, Dibyajyoti Nath6, Harun Gitari7, Daniel O. Wasonga8
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.052241
    (This article belongs to the Special Issue: Abiotic and Biotic Stress Tolerance in Crop)
    Abstract Agriculture plays a crucial role in the economy, and there is an increasing global emphasis on automating agricultural processes. With the tremendous increase in population, the demand for food and employment has also increased significantly. Agricultural methods traditionally used to meet these requirements are no longer adequate, requiring solutions to issues such as excessive herbicide use and the use of chemical fertilizers. Integration of technologies such as the Internet of Things, wireless communication, machine learning, artificial intelligence (AI), and deep learning shows promise in addressing these challenges. However, there is a lack of comprehensive documentation… More >

  • Open Access

    ARTICLE

    Response of Bacterial Community and Enzyme Activity of Greenhouse Tomato under Different Irrigation Systems

    Haijian Yang1, Mingzhi Zhang1,*, Na Xiao1, Yuan Li2
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.050915
    Abstract The micro-sprinkler irrigation mulched (MSM) has been suggested as a novel water-saving approach in controlled environment agriculture. However, the effects of microbial community structure and enzyme activity in the rhizosphere soil on crop growth under MSM remain unclear. This study conducted a randomized experimental design using greenhouse tomatoes to investigate changes in bacterial community structure and enzyme activity in rhizosphere soil under different irrigation frequencies (F) and amounts (I) of MSM. The findings revealed that with the increase of F or I, The total count of soil bacteria in tomatoes first rose and then fell… More >

  • Open Access

    ARTICLE

    Optimizing Sorghum Productivity Using Balanced Fertilizers on Dryland

    Samijan Samijan, Endah Nurwahyuni, Sri Minarsih*, Agus Supriyo, Sodiq Jauhari, Yulis Hindarwati, Meinarti Norma Setiapermas, Raden Heru Praptana, Endah Winarni, Vina Eka Aristya
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.048339
    (This article belongs to the Special Issue: Biofertilizers and Nano Fertilizers as Tools to Increase Agriculture Resilience and Sustainability)
    Abstract Sorghum is the fifth most required cereal crop globally. Sorghum bicolor has the advantage of being adaptive to both lowland and dryland, with drought-tolerant and wide adaptability. The low nutrient availability in dryland requires additional effective fertilizers to increase sorghum productivity. The research aimed to assess the application of organic, inorganic, and biofertilizers for sorghum productivity on dryland. Research in Central Java, Indonesia as dryland sorghum areas, from November 2022 to February 2023. The experiment cooperates with the farmers in a split-plot design, the main plot was two varieties and subplots of four fertilizers. The enhanced… More >

  • Open Access

    ARTICLE

    Enhancing Canola Yield and Photosynthesis under Water Stress with Hydrogel Polymers

    Elham A. Badr1, Gehan Sh. Bakhoum1, Mervat Sh. Sadak2, Ibrahim Al-Ashkar3, Mohammad Sohidul Islam4, Ayman El Sabagh5,6,*, Magdi T. Abdelhamid2,7,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.054453
    Abstract While Egypt’s canola production per unit area has recently grown, productivity remains low, necessitating increased productivity. Hydrogels are water-absorbent polymer compounds that can optimize irrigation schedules by increasing the soil’s ability to retain water. Accordingly, two field experiments were conducted to examine hydrogel application to sandy soil on canola growth, biochemical aspects, yield, yield traits, and nutritional quality of yielded seeds grown under water deficit stress conditions. The experiments were conducted by arranging a split-plot layout in a randomized complete block design (RCBD) with three times replications of each treatment. While water stress at 75%… More >

  • Open Access

    ARTICLE

    Comparative Chemical Research in Essential Oils from Six Apiaceae Species Growing in the Northern Region of Vietnam

    Nguyen Quang Hung1,2,#, Nguyen Thanh Tung3,#, Nguyen Phuong Hanh1, Chu Thi Thu Ha1, Nguyen Thi Nhung3, Nguyen Thai An3, Vu Xuan Giang3, Luong Van Hao4, Oleh Koshovyi5, Ain Raal5,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.053624
    Abstract Our study aimed to compare the essential oil (EO) concentration and composition of several Apiaceae species growing in the Northern region of Vietnam. The yields of EOs from materials ranged from 0.03% (root EO of Angelica acutiloba and aerial parts EO of Heracleum bivittatum)−0.27% (leaf EO of Xyloselinum vietnamense). Gas chromatography-mass spectrometry (GC-MS) allowed the identification of 74 components in the EOs of six Apiaceae species, making up 94.4%–100.0% of the oils. In EO from Angelica acutiloba, (Z)-ligustilide accounted for an extremely large proportion (94.9%). EO of Angelica pubescens was dominated by six characteristic components including α-pinene (21.5%), β-phellandrene (18.1%), p-cymene… More >

  • Open Access

    ARTICLE

    Experimental Exclusion of Guanaco Grazing Increases Cover, Diversity, Land Function and Plant Recruitment in Patagonia

    Carla Cepeda1,*, Gabriel Oliva1,2, Daniela Ferrante1,2
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.052534
    (This article belongs to the Special Issue: Ecology of Rangelands in Argentina)
    Abstract Semiarid Patagonia represents 25% of the rangeland area in Argentina, and sheep overgrazing has degraded about a third of it in the past. In this century, depleted domestic stocks have mostly stabilized, but Guanaco populations have grown. These native camelids share habitat and diets with sheep, but their effect on vegetation is poorly understood and has long been debated. We set up an exclusion experiment in Monte León and Cañadón Vaca, a semiarid shrubland grassland in southern Patagonia, currently grazed only by guanacos. Vegetation baselines were studied in 2016 in twelve plots, and half of… More >

  • Open Access

    ARTICLE

    Metabolomic Analysis of the Anthocyanins Associated with Different Colors of Cymbidium goeringii in Guizhou, China

    Dongbo Wu1,#, Shuang Qu2,#, Lianwen Shen2, Shengqun Chen2, Xia Jiang2,3, Ai Rao1, Yuxue Zhao2,*, Yueyun Wang1,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.051652
    (This article belongs to the Special Issue: Plant Secondary Metabolism and Functional Biology)
    Abstract Cymbidium goeringii is an economically important ornamental plant, and flower color is one of the main features of C. goeringii that contributes to its high economic value. To clarify the molecular mechanisms underlying the role of anthocyanins in mediating differences in color among varieties, liquid chromatography–tandem mass spectrometry was used to perform anthocyanin-targeted metabolomics of seven C. goeringii varieties, including ‘Jin Qian Yuan’ (JQY), ‘Jin Xiu Qian Yuan’ (JXQY), ‘Miao Jiang Su Die’ (MJSD), ‘Qian Ming Su’ (QMS), ‘Shi Chan’ (SC), and ‘Yang Ming Su’ (YMS), as well as the C. goeringii. We detected 64 anthocyanins, including cyanidins, delphinidins,… More >

  • Open Access

    ARTICLE

    Summer Warming Limited Bud Output Drives a Decline in Daughter Shoot Biomass through Reduced Photosynthetis of Parent Shoots in Leymus chinensis Seedlings

    Song Gao1, Ruocheng Xu2, Lin Li3, Jiao Wang2, Nian Liu2, Johannes M. H. Knops4, Junfeng Wang2,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.051548
    (This article belongs to the Special Issue: Grassland Ecology in China under Global Change)
    Abstract Understanding how summer warming influences the parent and daughter shoot production in a perennial clonal grass is vital for comprehending the response of grassland productivity to global warming. Here, we conducted a simulated experiment using potted Leymus chinensis, to study the relationship between the photosynthetic activity of parent shoots and the production of daughter shoots under a whole (90 days) summer warming scenario (+3°C). The results showed that the biomass of parents and buds decreased by 25.52% and 33.45%, respectively, under warming conditions. The reduction in parent shoot biomass due to warming directly resulted from decreased… More >

  • Open Access

    ARTICLE

    Cu Stress-Induced Transcriptome Alterations in Sorghum and Expression Analysis of the Transcription Factor-Encoding Gene SbWRKY24

    Mingchuan Yang1, Jia Zheng2, Wenhui Yu1, Yanghua Li2, Yali Wang1, Zilu Zhang1, Zhenhui Kang1,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.051718
    Abstract Sorghum is not only an important bio-energy crop but also a vital raw material for brewing. Exogenous copper affects the growth and metabolism of crops in specific ways. This study identified 8475 differentially expressed genes (DEGs) by high-throughput transcriptome sequencing in the sorghum cultivar ‘Jinnuoliang 2’ after 24 h of treatment with 10 mM CuSO. Using GO analysis, 476 genes were functionally annotated, which were mainly related to catabolism and biosynthetic processes. Additionally, 90 pathways were annotated by employing the KEGG analysis. Among them, glutathione metabolism and peroxisome were induced, while photosynthesis, photosynthesis-antenna protein, and… More >