Open Access

ARTICLE

miR-142-5p Inhibits Cell Invasion and Migration by Targeting DNMT1 in Breast Cancer

Hui Li^*1, Han-Han Li^†1, Qian Chen^‡1, Yu-Yang Wang^‡, Chang-Chang Fan^‡, Yuan-Yuan Duan^‡, You Huang^‡, Hui-Min Zhang^‡, Jia-Peng Li^‡, Xiao-Yu Zhang^‡, Yuan Xiang^‡, Chao-Jiang Gu^‡, Li Wang^§, Xing-Hua Liao^‡, Tong-Cun Zhang^‡¶

* Department of Hematology and Nephrology, Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Hubei, P.R. China
† Nanjing Vazyme Biotech Co. Ltd., Nanjing, P.R. China
‡ College of Life Sciences and Health, Wuhan University of Science and Technology, Hubei, P.R. China
§ School of Resource and Environmental Engineering, Wuhan University of Science and Technology, Hubei, P.R. China
¶ Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education and Tianjin, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, P.R. China

Oncology Research 2020, 28(9), 885-897. https://doi.org/10.3727/096504021X16274672547967

Abstract

Abnormal cell proliferation caused by abnormal transcription regulation mechanism seems to be one of the reasons for the progression of breast cancer and also the pathological basis. MicroRNA-142-5p (miR-142-5p) is a low-expressed miRNA in breast cancer. The role of MKL-1’s regulation of DNMT1 in breast cancer cell proliferation and migration is still unclear. MKL-1 (myocardin related transcription factor A) can bind to the conserved cis-regulatory element CC (A/T) 6GG (called CarG box) in the promoter to regulate the transcription of miR-142-5p. The expressions of miR-142-5p and MKL-1 are positively correlated. In addition, it has been proved that DNMT1 is the target of miR-142-5p, which inhibits the expression of DNMT1 by targeting the 3¢-UTR of DNMT1, thereby forming a feedback loop and inhibiting the migration and proliferation of breast cancer. Our data provide important and novel insights into the MKL-1/miR-142-5p/DNMT1/maspin signaling pathway and may become a new idea for breast cancer diagnosis, treatment, and prognosis.

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