Open Access
ARTICLE
Inhibition of Long Noncoding RNA CRNDE Increases Chemosensitivity of Medulloblastoma Cells by Targeting miR-29c-3p
Xiao-hui Sun*, Wen-jie Fan†, Zong-jian An‡, Yong Sun‡
* Medical Department, Qingdao Infectious Disease Hospital, Qingdao, Shandong, P.R. China
† Department of Anesthesia, Qingdao Women and Children’s Hospital, Qingdao, Shandong, P.R. China
‡ Department of Pediatric Neurosurgery, Qingdao Women and Children’s Hospital, Qingdao, Shandong, P.R. China
Oncology Research 2020, 28(1), 95-102. https://doi.org/10.3727/096504019X15742472027401
Abstract
Long noncoding RNA CRNDE (CRNDE) recently emerged as a carcinogenic promoter in various cancers
including medulloblastoma. However, the functions and molecular mechanisms of CRNDE to the acquired
drug resistance of medulloblastoma are still unclear. The transcript levels of CRNDE were examined in four
medulloblastoma cell lines exposed to cisplatin treatment, and IC
50 values were calculated. Effects of CRNDE
knockdown or miR-29c-3p overexpression on cell viability, colony formation, apoptosis, migration, and invasion were assessed using the CCK-8, colony formation assay, flow cytometry, and Transwell assays, respectively. RNA pulldown and RNA-binding protein immunoprecipitation (RIP) were performed to confirm the
molecular interactions between CRNDE and miR-29c-3p involved in medulloblastoma cells. The in vivo role
of CRNDE knockdown or miR-29c-3p overexpression on tumor growth and apoptosis was evaluated in a
xenograft mouse model of human medulloblastoma. The transcript levels of lncRNA CRNDE were significantly higher in cisplatin-treated tumor cells with higher IC50 values. Depletion of CRNDE inhibited tumor cell
proliferation and colony formation, induced cell apoptosis, and suppressed migration and invasion in medulloblastoma cells. Moreover, overexpression of miR-29c-3p inhibited tumor cell proliferation and colony formation, migration, and invasion, and enhanced apoptosis and chemosensitivity to cisplatin. In addition, CRNDE
was found to act as a miR-29c-3p sponge. Furthermore, in vivo experiments showed the CRNDE/miR-29c-3p
interactions involved in medulloblastoma. Our study demonstrates that CRNDE acts as a critical mediator of
proliferation, apoptosis, migration, invasion, and resistance to chemotherapeutics via binding to and negatively
regulating miR-29c-3p in medulloblastoma cells. These results provide novel molecular targets for treatment
of medulloblastoma.
Keywords
Cite This Article
Sun, X., Fan, W., An, Z., Sun, Y. (2020). Inhibition of Long Noncoding RNA CRNDE Increases Chemosensitivity of Medulloblastoma Cells by Targeting miR-29c-3p.
Oncology Research, 28(1), 95–102.