Open Access
ARTICLE
miR-632 Promotes Laryngeal Carcinoma Cell Proliferation, Migration, and Invasion Through Negative Regulation of GSK3b
Zhong-xin Zhou*, Zu-ping Zhang†, Ze-zhang Tao*, Ting-zhao Tan‡
* Department of Otorhinolaryngology, Renmin Hospital of Wuhan University, Wuhan, Hubei, P.R. China
† Department of Otorhinolaryngology, Liaocheng People’s Hospital, Liaocheng, Shandong, P.R. China
‡ Department of Oncology, Liaocheng Tumor Hospital, Liaocheng, Shandong, P.R. China
Oncology Research 2020, 28(1), 21-31. https://doi.org/10.3727/096504018X15213142076069
Abstract
Laryngeal cancer, one of the most common head and neck malignancies, is an aggressive neoplasm. Increasing
evidence has demonstrated that microRNAs (miRNAs) exert important roles in oncogenesis and progression
of diverse types of human cancers. miR-632, a tumor-related miRNA, has been reported to be dysregulated
and implicated in human malignancies; however, its biological role in laryngeal carcinoma remains to be elucidated. The present study aimed at exploring the role of miR-632 in laryngeal cancer and clarifying the potential
molecular mechanisms involved. In the current study, miR-632 was found to be significantly upregulated
both in laryngeal cancer tissues and laryngeal cancer cell lines. Functional studies demonstrated that miR-632
accelerated cell proliferation and colony formation, facilitated cell migration and invasion, and enhanced the
expression of cell proliferation-associated proteins, cyclin D1 and c-myc. Notably, miR-632 could directly
bind to the 3 -untranslated region (3 -UTR) of glycogen synthase kinase 3 (GSK3 ) to suppress its expression
in laryngeal cancer cells. Mechanical studies revealed that miR-632 promoted laryngeal cancer cell proliferation, migration, and invasion through negative modulation of GSK3 . Pearson’s correlation analysis revealed
that miR-632 expression was inversely correlated with GSK3 mRNA expression in laryngeal cancer tissues.
Taken together, our findings suggest that miR-632 functions as an oncogene in laryngeal cancer and may be
used as a novel therapeutic target for laryngeal cancer.
Keywords
Cite This Article
Zhou, Z., Zhang, Z., Tao, Z., Tan, T. (2020). miR-632 Promotes Laryngeal Carcinoma Cell Proliferation, Migration, and Invasion Through Negative Regulation of GSK3b.
Oncology Research, 28(1), 21–31.