Open Access

ARTICLE

miR-185 Inhibits the Proliferation and Invasion of Non-Small Cell Lung Cancer by Targeting KLF7

Lili Zhao^*, Yao Zhang^*, Jiaoxia Liu^*, Wei Yin^†, Dan Jin^‡, Dandan Wang^*, Wei Zhang^*

* Affiliated Hongqi Hospital of Mudanjiang Medical University, Mudanjiang City, P.R. China
† Mudanjiang Forestry Hospital, Mudanjiang City, P.R. China
‡ Mudanjiang Women and Children’s hospital, Mudanjiang City, P.R. China

Oncology Research 2019, 27(9), 1015-1023. https://doi.org/10.3727/096504018X15247341491655

Abstract

MicroRNAs (miRNAs) are short endogenous noncoding RNAs that frequently play vital roles in many cancer types. Herein we demonstrated that miR-185 was remarkably downregulated in NSCLC tissues compared with adjacent normal tissues. A lower level of miR-185 was associated with lymph node metastasis. Functional assays showed that upregulation of miR-185 inhibited the proliferation, colony formation, and invasion capacities of NSCLC cells in vitro. Furthermore, we found that miR-185 suppressed the epithelial–mesenchymal transition (EMT) process. Bioinformatics analysis and luciferase reporter gene assays revealed that Kruppellike factor 7 (KLF7) was the target of miR-185. Overexpression of miR-185 reduced the expression of KLF7 in NSCLC cells. Upregulation of KLF7 partly neutralized the inhibitory effects of miR-185 on the proliferation and invasion of NSCLC. Additionally, we confirmed that miR-185 suppressed the tumor growth of NSCLC A549 cells in vivo. Taken together, these results demonstrate that miR-185 acts as a suppressor by targeting KLF7 in NSCLC.

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