Open Access
ARTICLE
Exosomal miR-1228 From Cancer-Associated Fibroblasts Promotes Cell Migration and Invasion of Osteosarcoma by Directly Targeting SCAI
Jian-Wei Wang, Xiao-Feng Wu, Xiao-Juan Gu, Xing-Hua Jiang
Department of Orthopedic, Kunshan Hospital of Traditional Chinese Medicine, Kunshan, Jiangsu, P.R. China
Oncology Research 2019, 27(9), 979-986. https://doi.org/10.3727/096504018X15336368805108
Abstract
Cancer-associated fibroblasts (CAFs) play a predominant role in regulating tumor progression. Understanding
how CAFs communicate with osteosarcoma is crucial for developing novel approaches for osteosarcoma therapy. Exosomes are able to transmit messages between cells. In this study, we demonstrated that CAFs transfer
exosomes to osteosarcoma cells, which promotes osteosarcoma cell migration and invasion. Using a miRNA
microarray analysis, we identified 13 miRNAs that are significantly increased in exosomes derived from
cancer-associated fibroblasts (CAFs) and corresponding paracancer fibroblasts (PAFs). In vitro studies further validated that the levels of microRNA-1228 (miR-1228) were increased in CAFs, its secreted exosomes,
and in recipient osteosarcoma cells, which can downregulate endogenous SCAI mRNA and protein level in
osteosarcoma. Furthermore, our findings demonstrate that SCAI was downregulated in osteosarcoma tissues.
Taken together, this study provides evidence that CAF exosomal miR-1228 is able to promote osteosarcoma
invasion and migration by targeting SCAI, which may represent a critical therapeutic target for osteosarcoma
treatment.
Keywords
Cite This Article
Wang, J., Wu, X., Gu, X., Jiang, X. (2019). Exosomal miR-1228 From Cancer-Associated Fibroblasts Promotes Cell Migration and Invasion of Osteosarcoma by Directly Targeting SCAI.
Oncology Research, 27(9), 979–986. https://doi.org/10.3727/096504018X15336368805108