Open Access

ARTICLE

MicroRNA-152 Acts as a Tumor Suppressor MicroRNA by Inhibiting Krüppel-Like Factor 5 in Human Cervical Cancer

Haiyan Zhang^*†, Yanxia Lu^‡, Surong Wang^‡, Xiugui Sheng^§, Shiqian Zhang^*

* Department of Gynecology, Affiliated Qilu Hospital of Shandong University, Lixia District, Jinan, Shandong, P.R. China
† Department of Gynecology Ward-1, Linyi People’s Hospital, Hedong District, Linyi, Shandong, P.R. China
‡ Department of Gynecology Ward-3, Linyi People’s Hospital, Linyi, Hedong District, Linyi, Shandong, P.R. China
§ Department of Gynecology, Cancer Hospital Chinese Academy of Medical Sciences, Chaoyang District, Beijing, P.R. China

Oncology Research 2019, 27(3), 335-340. https://doi.org/10.3727/096504018X15252202178408

Abstract

Aberrant expression of microRNA-152 (miR-152) is frequently observed in human cancers including ovarian cancer, breast cancer, prostate cancer, and gastric cancer. However, its expression and functional role in cervical cancer (CC) are poorly understood. Also, the association between miR-152 and Krüppel-like factor 5 (KLF5) expression in CC remains unclear. In this study, analyzing the expression of miR-152 by quantitative real-time PCR (qRT-PCR) revealed it was sharply reduced in CC tissues and cell lines. In addition, the negative correlation of miR-152 expression and KLF5 expression was observed. The dual-luciferase reporter assay validated that KLF5 was a target of miR-152. In vitro functional assays revealed that miR-152 could inhibit cell proliferation and cell cycle progression through regulating the expression of KLF5. Taken together, our study suggested that miR-152 functions as a tumor suppressor in CC, and the miR-152/KLF5 axis may provide novel therapeutic targets for CC treatment.

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