Open Access

ARTICLE

miR-150 Suppresses Tumor Growth in Melanoma Through Downregulation of MYB

Xiyan Sun^*1, Chao Zhang^†1, Yang Cao^†, Erbiao Liu^*

* Department of Oncology, Shanxian Central Hospital, Heze, Shandong Province, P.R. China
† Department of Dermatology, Shanxian Central Hospital, Heze, Shandong Province, P.R. China

Oncology Research 2019, 27(3), 317-323. https://doi.org/10.3727/096504018X15228863026239

Abstract

miR-150 has been demonstrated to inhibit tumor progression in various human cancers, including colorectal cancer, ovarian cancer, and thyroid cancer. However, the role of miR-150 in melanoma remains to be determined. In this study, we found that miR-150 was underexpressed in melanoma tissues and cell lines. Through transfection of miR-150 mimics, we found that miR-150 significantly inhibited the proliferation, migration, and invasion of melanoma cells. In mechanism, we found that MYB was a target of miR-150 in melanoma cells. Overexpression of miR-150 significantly inhibited mRNA and protein levels of MYB in melanoma cells. Moreover, there was an inverse correlation between the expression of miR-150 and MYB in melanoma tissues. We also showed that MYB was upregulated in melanoma tissues and cell lines. Through functional experiments, we found that restoration of MYB in miR-150-overexpressed melanoma cells rescued the proliferation, migration, and invasion. Therefore, our findings demonstrated that miR-150 suppressed the proliferation, migration, and invasion of melanoma cell by downregulating MYB.

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