Open Access
ARTICLE
MicroRNA 615-3p Inhibits the Tumor Growth and Metastasis of NSCLC via Inhibiting IGF2
Jiangtao Liu*, Yanli Jia*, Lijuan Jia*, Tingting Li†, Lei Yang‡, Gongwen Zhang‡
* Medical Oncology, Binzhou Central Hospital, Binzhou, Shandong, P.R. China
† Department of Anesthesiology, Binzhou Central Hospital, Binzhou, Shandong, P.R. China
‡ Department of Cardiothoracic Surgery, Binzhou Central Hospital, Binzhou, Shandong, P.R. China
Oncology Research 2019, 27(2), 269-279. https://doi.org/10.3727/096504018X15215019227688
Abstract
MicroRNAs are essential regulators of cancer-associated genes at the posttranscriptional level, and their
expression is altered in cancer tissues. Herein we sought to identify the regulation of miR-615-3p in NSCLC
progression and its mechanism. miR-615-3p expression was significantly downregulated in NSCLC tissue
compared to control normal tissue. Exogenous overexpression of miR-615-3p inhibited the growth and metastasis of NSCLC cells. In addition, the in vivo mouse xenograft model showed that overexpression of miR-
615-3p inhibited NSCLC growth and lung metastasis, whereas decreased expression of miR-615-3p caused an
opposite outcome. Furthermore, we revealed that insulin-like growth factor 2 (IGF2) expression was negatively
correlated with the miR-615-3p level in NSCLC specimens, and IGF2 knockdown mimicked the effect of miR-
615-3p inhibition on NSCLC cell proliferation, migration, and invasion. In addition, overexpression of IGF2
rescued the inhibition of miR-615-3p in NSCLC cells. Together, our results indicated that miR-615-3p played
important roles in the regulation of NSCLC growth and metastasis by targeting IGF2.
Keywords
Cite This Article
Liu, J., Jia, Y., Jia, L., Li, T., Yang, L. et al. (2019). MicroRNA 615-3p Inhibits the Tumor Growth and Metastasis of NSCLC via Inhibiting IGF2.
Oncology Research, 27(2), 269–279.