Open Access

ARTICLE

MicroRNA 615-3p Inhibits the Tumor Growth and Metastasis of NSCLC via Inhibiting IGF2

Jiangtao Liu^*, Yanli Jia^*, Lijuan Jia^*, Tingting Li^†, Lei Yang^‡, Gongwen Zhang^‡

* Medical Oncology, Binzhou Central Hospital, Binzhou, Shandong, P.R. China
† Department of Anesthesiology, Binzhou Central Hospital, Binzhou, Shandong, P.R. China
‡ Department of Cardiothoracic Surgery, Binzhou Central Hospital, Binzhou, Shandong, P.R. China

Oncology Research 2019, 27(2), 269-279. https://doi.org/10.3727/096504018X15215019227688

Abstract

MicroRNAs are essential regulators of cancer-associated genes at the posttranscriptional level, and their expression is altered in cancer tissues. Herein we sought to identify the regulation of miR-615-3p in NSCLC progression and its mechanism. miR-615-3p expression was significantly downregulated in NSCLC tissue compared to control normal tissue. Exogenous overexpression of miR-615-3p inhibited the growth and metastasis of NSCLC cells. In addition, the in vivo mouse xenograft model showed that overexpression of miR- 615-3p inhibited NSCLC growth and lung metastasis, whereas decreased expression of miR-615-3p caused an opposite outcome. Furthermore, we revealed that insulin-like growth factor 2 (IGF2) expression was negatively correlated with the miR-615-3p level in NSCLC specimens, and IGF2 knockdown mimicked the effect of miR- 615-3p inhibition on NSCLC cell proliferation, migration, and invasion. In addition, overexpression of IGF2 rescued the inhibition of miR-615-3p in NSCLC cells. Together, our results indicated that miR-615-3p played important roles in the regulation of NSCLC growth and metastasis by targeting IGF2.

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