Open Access
ARTICLE
miR-449a Suppresses Tumor Growth, Migration, and Invasion in Non-Small Cell Lung Cancer by Targeting a HMGB1-Mediated NF-kB Signaling Pathway
Dandan Wu*1,
Jun Liu†1,
Jianliang Chen*, Haiyan He*, Hang Ma*, Xuedong Lv*
* Department of Respiratory Medicine, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, P.R. China
† Department of Cardiology, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, P.R. China
Oncology Research 2019, 27(2), 227-235. https://doi.org/10.3727/096504018X15213089759999
Abstract
MicroRNAs (miRNAs) have been reported to be involved in many human cancers and tumor progression. The
dysregulation of miR-449a is found in many types of malignancies and is associated with tumor growth, migration, and invasion. However, its expression and function in non-small cell lung cancer (NSCLC) still remains
unclear. In our study, miR-449a was found to be downregulated in both NSCLC tissues and cell lines, and
low miR-449a expression was obviously associated with tumor differentiation, TMN stage, and poor overall
survival (OS). Moreover, we demonstrated that miR-449a could inhibit tumor proliferation, migration, and
invasion in NSCLC. We also confirmed that HMGB1 was a direct target gene of miR-449a in NSCLC with
dual-luciferase reporter assay, and upregulation of HMGB1 could reverse the miR-449a-induced suppression
of growth, migration, and invasion in NSCLC cells. Last, we found that miR-449a suppressed tumor initiation
and development through the NF- B signaling pathway. These results indicate that miR-449a functions as a
tumor suppressor in NSCLC by targeting the HMGB1-mediated NF- B signaling pathway in NSCLC.
Keywords
Cite This Article
Wu, D., Liu,
. J., Chen,
. J., He, H., Ma, H. et al. (2019). miR-449a Suppresses Tumor Growth, Migration, and Invasion in Non-Small Cell Lung Cancer by Targeting a HMGB1-Mediated NF-kB Signaling Pathway.
Oncology Research, 27(2), 227–235.