Open Access
ARTICLE
MicroRNA-338-3p Inhibits Proliferation and Promotes Apoptosis of Multiple Myeloma Cells Through Targeting Cyclin-Dependent Kinase 4
Yang Cao*, Xu Shi†, Yingmin Liu‡, Ren Xu§, Qing Ai*
* Clinical Laboratory, the First Affiliated Hospital, Jilin University, Chaoyang District, Changchun, P.R. China
† Central Laboratory, the First Affiliated Hospital, Jilin University, Chaoyang District, Changchun, P.R. China
‡ Department of Hematology Cancer Center, the First Affiliated Hospital, Jilin University, Chaoyang District,
Changchun, P.R. China
§ Department of Respiratory, the First Affiliated Hospital, Jilin University, Chaoyang District,
Changchun, P.R. China
Oncology Research 2019, 27(1), 117-124. https://doi.org/10.3727/096504018X15213031799835
Abstract
MicroRNA-338-3p (miR-338-3p) has been reported to be a tumor suppressor in multiple cancer types.
However, the biological role of miR-338-3p and its underlying mechanism in multiple myeloma (MM)
remain unclear. In the present study, we investigated the biological role and potential of miR-338-3p in
MM. We found that miR-338-3p was significantly decreased in newly diagnosed and relapsed MM tissues and cell lines. Overexpression of miR-338-3p in MM cells significantly inhibited proliferation and
promoted apoptosis, caspase 3, and caspase 8 activity. Bioinformatics algorithm analysis predicted that
cyclin-dependent kinase 4 (CDK4) was a direct target of miR-338-3p, and this was experimentally verified
by a dual-luciferase reporter assay. Furthermore, overexpression of miR-338-3p inhibited CDK4 expression
on mRNA and protein levels. Of note, the restoration of CDK4 expression markedly abolished the effect of
miR-338-3p overexpression on cell proliferation, apoptosis, caspase 3, and caspase 8 activities in MM cells.
Taken together, the present study is the first to demonstrate that miR-338-3p functions as a tumor suppressor
in MM through inhibiting CDK4. This finding implies that miR-338-3p is a potential therapeutic target for
the treatment of MM.
Keywords
Cite This Article
Cao, Y., Shi, X., Liu, Y., Xu, R., Ai, Q. (2019). MicroRNA-338-3p Inhibits Proliferation and Promotes Apoptosis of Multiple Myeloma Cells Through Targeting Cyclin-Dependent Kinase 4.
Oncology Research, 27(1), 117–124. https://doi.org/10.3727/096504018X15213031799835