Open Access

ARTICLE

Knockdown of Long Noncoding RNA CAT104 Inhibits the Proliferation, Migration, and Invasion of Human Osteosarcoma Cells by Regulating MicroRNA-381

Bo Xia^*, Lei Wang^†, Li Feng^*, Baofang Tian^*, Yuanjie Tan^‡, Baoyin Du^*

* Department of Emergency Trauma Surgery, Jining No. 1 People’s Hospital, Jining, Shandong, P.R. China
† Second Department of Orthopedics, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, P.R. China
‡ Department of Cardiology, Weihai Hospital of Traditional Chinese Medicine, Weihai, Shandong, P.R. China

Oncology Research 2019, 27(1), 89-98. https://doi.org/10.3727/096504018X15199511344806

Retracted 17 July 2024

Abstract

Osteosarcoma is the most common primary malignant bone tumor in children and adolescents. This study aimed to explore the effects of long noncoding RNA CAT104 and microRNA-381 (miR-381) on osteosarcoma cell proliferation, migration, invasion, and apoptosis, as well as the underlying potential mechanism. We found that CAT104 was highly expressed in osteosarcoma MG63 and OS-732 cells. Knockdown of CAT104 significantly inhibited OS-732 cell proliferation, migration, and invasion, but promoted cell apoptosis. CAT104 regulated the expression of miR-381, and miR-381 participated in the effects of CAT104 on OS-732 cells. Zinc finger E-box-binding homeobox 1 (ZEB1) was a direct target gene of miR-381, which was involved in the regulatory roles of miR-381 in OS-732 cell proliferation, migration, invasion, and apoptosis, as well as c-Jun N-terminal kinase (JNK) and Wnt/ -catenin pathways. In conclusion, our research verified that suppression of CAT104 exerted significant inhibitory effects on osteosarcoma cell proliferation, migration, and invasion by regulating the expression of miR-381 and downstream ZEB1, as well as JNK and Wnt/ -catenin pathways.

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Keywords

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