Open Access
ARTICLE
Knockdown of Urothelial Carcinoma-Associated 1 Suppressed Cell Growth and Migration Through Regulating miR-301a and CXCR4 in Osteosarcoma MHCC97 Cells
Genglong Zhu*, Xialei Liu*, Yonghui Su†, Fangen Kong‡, Xiaopeng Hong*, Zhidong Lin†
* Department of Hepatobiliary Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, P.R. China
† Department of General Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, P.R. China
‡ Department of Neurosurgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, P.R. China
Oncology Research 2019, 27(1), 55-64. https://doi.org/10.3727/096504018X15201143705855
Retracted 17 July 2024
A retraction of this article was approved in:
Retraction: Knockdown of Urothelial Carcinoma-Associated 1 Suppressed Cell Growth and Migration Through Regulating miR-301a and CXCR4 in Osteosarcoma MHCC97 Cells
Read retraction
Abstract
Liver cancer is one of the most common malignancies in the world and a leading cause of cancer-related mortality. Accumulating evidence has highlighted the critical role of long noncoding RNAs (lncRNAs) in various
cancers. The present study aimed to explore the role of lncRNA urothelial carcinoma-associated 1 (UCA1) in
cell growth and migration in MHCC97 cells and its underlying mechanism. First, we assessed the expression
of UCA1 in MHCC97 and three other cell lines by RT-qPCR. Then the expression of UCA1, miR-301a, and
CXCR4 in MHCC97 cells was altered by transient transfection. The effects of UCA1 and miR-301 on cell
viability, migration, invasion, and apoptosis were assessed. The results revealed that UCA1 expression was
relatively higher in MHCC97 cells than in MG63, hFOB1.19, and OS-732 cells. Knockdown of UCA1 reduced
cell viability, inhibited migration and invasion, and promoted cell apoptosis. However, the effect of UCA1
knockdown on cell growth and migration was blocked by miR-301a overexpression, whose expression was
regulated by UCA1. We also found that miR-301a positively regulated CXCR4 expression. CXCR4 inhibition
reversed the effect of miR-301a overexpression on cell growth and migration. Moreover, miR-301a activated
the Wnt/ -catenin and NF- B pathways via regulating CXCR4. The present study demonstrated that UCA1
inhibition exerted an antigrowth and antimigration role in MHCC97 cells through regulating miR-301a and
CXCR4 expression.
Keywords
Cite This Article
APA Style
Zhu, G., Liu, X., Su, Y., Kong, F., Hong, X. et al. (2019). Knockdown of urothelial carcinoma-associated 1 suppressed cell growth and migration through regulating mir-301a and CXCR4 in osteosarcoma MHCC97 cells. Oncology Research, 27(1), 55-64. https://doi.org/10.3727/096504018X15201143705855
Vancouver Style
Zhu G, Liu X, Su Y, Kong F, Hong X, Lin Z. Knockdown of urothelial carcinoma-associated 1 suppressed cell growth and migration through regulating mir-301a and CXCR4 in osteosarcoma MHCC97 cells. Oncol Res. 2019;27(1):55-64 https://doi.org/10.3727/096504018X15201143705855
IEEE Style
G. Zhu, X. Liu, Y. Su, F. Kong, X. Hong, and Z. Lin "Knockdown of Urothelial Carcinoma-Associated 1 Suppressed Cell Growth and Migration Through Regulating miR-301a and CXCR4 in Osteosarcoma MHCC97 Cells," Oncol. Res., vol. 27, no. 1, pp. 55-64. 2019. https://doi.org/10.3727/096504018X15201143705855