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Propofol Inhibits Lung Cancer A549 Cell Growth and Epithelial–Mesenchymal Transition Process by Upregulation of MicroRNA-1284
Wei-Zhen Liu, Nian Liu
Department of Anesthesia, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine,
Central South University, Changsha, P.R. China
Oncology Research 2019, 27(1), 1-8. https://doi.org/10.3727/096504018X15172738893959
Retracted 17 July 2024
A retraction of this article was approved in:
Retraction: Propofol Inhibits Lung Cancer A549 Cell Growth and Epithelial-Mesenchymal Transition Process by Upregulation of MicroRNA-1284
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Abstract
Propofol has been widely used in lung cancer resections. Some studies have demonstrated that the effects
of propofol might be mediated by microRNAs (miRNAs). This study aimed to investigate the effects and
mechanisms of propofol on lung cancer cells by regulation of miR-1284. A549 cells were treated with different
concentrations of propofol, while transfected with miR-1284 inhibitor, si-FOXM1, and their negative controls.
Cell viability, migration, and invasion, and the expression of miR-1284, FOXM1, and epithelial–mesenchymal
transition (EMT) factors were detected by CCK-8, Transwell, qRT-PCR, and Western blot assays, respectively.
In addition, the regulatory and binding relationships among propofol, miR-1284, and FOXM1 were assessed,
respectively. Results showed that propofol suppressed A549 cell viability, migration, and invasion, upregulated
E-cadherin, and downregulated N-cadherin, vimentin, and Snail expressions. Moreover, propofol significantly
promoted the expression of miR-1284. miR-1284 suppression abolished propofol-induced decreases of cell
viability, migration, and invasion, and increased FOXM1 expression and the luciferase activity of FOXM1-wt.
Further, miR-1284 negatively regulated FOXM1 expression. FOXM1 knockdown reduced cell viability, migration, and invasion by propofol treatment plus miR-1284 suppression. In conclusion, our study indicated that
propofol could inhibit cell viability, migration, invasion, and the EMT process in lung cancer cells by regulation of miR-1284.
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APA Style
Liu, W., Liu, N. (2019). Propofol inhibits lung cancer A549 cell growth and epithelial–mesenchymal transition process by upregulation of microrna-1284. Oncology Research, 27(1), 1-8. https://doi.org/10.3727/096504018X15172738893959
Vancouver Style
Liu W, Liu N. Propofol inhibits lung cancer A549 cell growth and epithelial–mesenchymal transition process by upregulation of microrna-1284. Oncol Res. 2019;27(1):1-8 https://doi.org/10.3727/096504018X15172738893959
IEEE Style
W. Liu and N. Liu, "Propofol Inhibits Lung Cancer A549 Cell Growth and Epithelial–Mesenchymal Transition Process by Upregulation of MicroRNA-1284," Oncol. Res., vol. 27, no. 1, pp. 1-8. 2019. https://doi.org/10.3727/096504018X15172738893959