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Shilei Wang^*, Yuzuo Hui^*, Xiaoming Li^†, Qingbin Jia^*
Oncology Research, Vol.26, No.8, pp. 1143-1154, 2018, DOI:10.3727/096504017X14965095236521
Abstract Gliomas are the most common primary brain tumors with high mortality. The treatment for gliomas is largely limited due to its uncomprehending pathological mechanism. Here we aimed to investigate the effect of long noncoding RNA (lncRNA) coiled-coil domain-containing 26 (CCDC26) in glioma progression. In our study, the expression of CCDC26 was found upregulated in glioma tissues and cell lines compared with normal tissues and cell lines. Further exploration detected decreased cell proliferation and increased cell apoptosis in U-251 and M059J cells transfected with CCDC26-siRNA. In addition, the silencing of CCDC26 strongly reduced the wound closing… More >

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Jie Shen^*, Jianhua Zhang^†, Minhui Xiao^*, Junfeng Yang^*, Ningnan Zhang^*
Oncology Research, Vol.26, No.8, pp. 1155-1165, 2018, DOI:10.3727/096504017X15041934685237
Abstract miR-203 is an epigenetically silenced tumor-suppressive microRNA in tumors. This study was designed to investigate the effects of miR-203 on the proliferation, migration, invasion, and apoptosis of bladder cancer (BCa) cells. The expression levels of miR-203 in BCa tissues, normal adjacent tissues, and BCa cell lines were detected. BCa cells were transfected with miR-203 mimic and inhibitor to investigate the effect of miR-203 on cell functions and the epithelial–mesenchymal transition (EMT). Cotransfection with miR-203 inhibitor and shRNA of the predicted target gene Twist1 (si-Twist1) was performed to investigate the target relationship of miR-203 and Twist1.… More >

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Qingzhu Ma^*, Yan Wang^†, Hualing Zhang^*, Fengqiang Wang^†
Oncology Research, Vol.26, No.8, pp. 1167-1174, 2018, DOI:10.3727/096504017X15051741798389
Abstract Colorectal cancer (CRC) is one of the most common oncological conditions worldwide, to date. MicroRNA- 1290 (miR-1290) has been demonstrated to regulate its progression. We studied the role of miR-1290 in CRC progression. The gene was upregulated in CRC tissues and cells. Its overexpression promoted CRC cell proliferation analyzed by MTT assay, colony formation assay, and soft agar growth assay. In addition, miR-1290 knockdown inhibited CRC cell proliferation. We also found that miR-1290 overexpression reduced the p27 level and increased cyclin D1 at both the mRNA and protein levels, whereas miR-1290 knockdown increased p27 and More >

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Hung-Tsung Lee^*, Cheng-Hsieh Huang^†, Wuan-Chun Chen^†, Chi-Shan Tsai^†, Yu-Lin Chao^†, Szu-Han Liu^†, Jun-Hong Chen^†, Yi-Ying Wu^‡, Yi-Ju Lee^†§
Oncology Research, Vol.26, No.8, pp. 1175-1182, 2018, DOI:10.3727/096504018X15149761920868
Abstract Lung cancer is the leading cause of cancer deaths worldwide. Given that the major threat of cancer is metastasis, delineation of the molecular mechanism underlying it would help devise therapeutic strategies. Transglutaminase 2 (TG2), belonging to the transglutaminase superfamily, is a versatile protein with enzymatic and nonenzymatic functions. It mainly localizes inside the cell, but also appears extracellularly. Recent findings have demonstrated the involvement of TG2 in cancer development. Here we examine the role of TG2 in metastasis of lung cancer using a lung cancer cell line CL1-0, which exhibits low invasiveness, and its invasive… More >

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Geqiong Xiao^*, Qiong Wang^*, Bo Li^†, Xiaohui Wu^‡, Hui Liao^*, Yili Ren^*, Ning Ai^†*
Oncology Research, Vol.26, No.8, pp. 1183-1189, 2018, DOI:10.3727/096504018X15151495109394
Abstract Recent studies have revealed abnormal expression of miRNAs in various tumors. Although microRNA-338-3p (miR-338-3p) plays an important role in many types of tumors, its influence on liver cancer (LC) is unknown. In this study, we found that expression of miR-338-3p was decreased in LC cells and tissues. Colony formation and cell proliferation were suppressed by enhanced expression of miR-338-3p in LC cells. Moreover, miR-338-3p targeted sphingosine kinase 2 (SphK2). Silencing of SphK2 had an identical influence as overexpression of miR-338-3p in LC cells. Overexpression of SphK2 without the 3'-untranslated region remarkably enhanced the growth suppression More >

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Tingting Zhang^*1, Ning Wang^†1
Oncology Research, Vol.26, No.8, pp. 1191-1200, 2018, DOI:10.3727/096504018X15166204902353
Abstract The EGFR tyrosine kinase inhibitor gefitinib is used in therapy for non-small cell lung cancer (NSCLC). However, the therapeutic efficacy of gefitinib is known to be impeded by mutations of EGFR. The aim of the present study was to reveal the role of miR-135a in gefitinib resistance of NSCLC cells. Human NSCLC cell lines, NCI-H1650 and NCI-H1975, were transfected with miR-135a mimic/inhibitor or miR-135a inhibitor plus pEX-RAC1 (a RAC1-expressing vector). The effects of miR-135a and RAC1 expression on cell viability, apoptosis, migration, and invasion were then detected. The transfected cells were exposed to 0–20 µM… More >

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Bui Thi Kim Ly^*, Hoang Thanh Chi^†‡
Oncology Research, Vol.26, No.8, pp. 1201-1205, 2018, DOI:10.3727/096504018X15154104709325
Abstract FMS-like tyrosine kinase-3 fragments from exon 14 to the end without any mutations or deletions have been reported to fuse to ETV6 (TEL) in a few cases of myeloid/lymphoid neoplasms with eosinophilia carrying a translocation t(12;13)(p13;q12). This fusion protein confers constitutive activation on the FLT3 fragment and induces factor-independent growth in transfected Ba/F3 cells, indicating that it is an oncoprotein. However, the mechanism controlling the stability of this oncoprotein is unknown. In this study, we focus on finding factors controlling the stability of ETV6/FLT3. We have shown that the stability of ETV6/FLT3 is regulated by… More >

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Junxun Ma, Lijie Wang, Jinyu Li, Guoqing Zhang, Haitao Tao, Xiaoyan Li, Danyang Sun, Yi Hu
Oncology Research, Vol.26, No.8, pp. 1207-1213, 2018, DOI:10.3727/096504017X15046134836575
Abstract Esophageal cancer is a common gastrointestinal cancer, with a very high mortality rate in patients with metastasis. Swainsonine, a cytotoxic fungal alkaloid, has been shown to inhibit cell growth in esophageal cancer. In the present study, we explored the effects of swainsonine on cell invasion and metastasis in esophageal cancer cells. Human esophageal carcinoma cells were treated with different doses of swainsonine, and then cell viability, invasion, and apoptosis were measured. The mRNA and protein expressions of Twist1, apoptosis- and EMT-related factors, and PI3K/AKT pathway factors were detected by qRT-PCR and Western blot. Swainsonine had More >

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Jing Feng
Oncology Research, Vol.26, No.8, pp. 1215-1225, 2018, DOI:10.3727/096504017X15021536183526
Abstract More and more studies have reported that dysregulation of microRNAs (miRNAs) leads to the proliferation and EMT of multiple cancers. Recently, several reports have demonstrated that dysregulation of miR-4262 occurs in numerous cancers. However, its role and precise mechanism in human cervical cancer (CC) have not been well clarified. Hence, this study aimed to explore the biological roles and precise mechanisms of miR-4262 in CC cell lines. The level of miR-4262 was found to be significantly decreased in CC tissues and cell lines. Moreover, decreased expression of miR-4262 was closely related to increased expression of More >

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Xiaoyun Liu¹, Shuguang Li¹, Yanyan Li, Bo Cheng, Bo Tan, Gang Wang
Oncology Research, Vol.26, No.8, pp. 1227-1234, 2018, DOI:10.3727/096504018X15178736525106
Abstract Bladder cancer (BC) is a common disease of the urinary system. Puerarin is a flavonoid extracted from Pueraria lobata. However, the role of puerarin in BC remains unclear. Hence, this study aimed to investigate the effect of puerarin on BC cells. Cell viability, proliferation, and apoptosis were measured by CCK-8, BrdU assay, and flow cytometry analysis, respectively. The expressions of miR-16, apoptosis-related factors, and the main factors of the NF-kB pathway were analyzed by qRT-PCR and Western blot. In this study, we found that cell viability and proliferation were significantly reduced, cell apoptosis was enhanced, and… More >

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Jian Jiang^*, Xuewen Song^†, Jing Yang^*, Ke Lei^*, Yongan Ni^*, Fei Zhou^‡, Lirong Sun^*
Oncology Research, Vol.26, No.8, pp. 1235-1243, 2018, DOI:10.3727/096504018X15179661552702
Abstract Neuroblastoma is the primary cause of cancer-related death for children 1 to 5 years of age. New therapeutic strategies and medicines are urgently needed. This study aimed to investigate the effects of triptolide (TPL), the major active component purified from Tripterygium wilfordii Hook F, on neuroblastoma SH-SY5Y cell proliferation, migration, and apoptosis, as well as underlying potential mechanisms. We found that TPL inhibited SH-SY5Y cell viability, proliferation, and migration, but induced cell apoptosis. The expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 after TPL treatment in SH-SY5Y cells was decreased. The expression of microRNA-181a (miR-181a) was upregulated More >

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Ming Zhang^*, Change Gao^†, Yi Yang^*, Gaofeng Li^‡, Jian Dong^§, Yiqin Ai^*, Nan Chen^‡, Wenhui Li^*
Oncology Research, Vol.26, No.8, pp. 1245-1255, 2018, DOI:10.3727/096504017X14944585873668
Abstract Long noncoding RNAs (lncRNAs), a new class of functional regulators involved in human tumorigenesis, have been attracting the increasing attention of researchers. The lncRNA colorectal neoplasia differentially expressed (CRNDE) gene, transcribed from chromosome 16 on the strand opposite the adjacent IRX5 gene, was originally found to be increased in CRC and was reported to be abnormally expressed in many cancers. However, its potential role and the molecular mechanism underlying the radioresistant phenotype formation of lung adenocarcinoma (LAD) remain unclear. In our present study, we identified that CRNDE was significantly upregulated in LAD tissue and radioresistant… More >

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Juhua Zhuang^*1, Saifei He^*1, Guoyu Wang^*, Guangdong Wang^*†, Jing Ni^*, Suiliang Zhang^‡, Ying Ye^*, Wei Xia^*
Oncology Research, Vol.26, No.8, pp. 1257-1265, 2018, DOI:10.3727/096504018X15172756878992
Abstract Hepatocellular carcinoma (HCC) as one of the most refractory cancers leads to high mortality worldwide. Long noncoding RNAs have been widely acknowledged as important biomarkers and therapeutic targets in HCC. In this study, we investigated the effects of long noncoding RNA FGFR3-AS1 on tumor growth and metastasis in HCC. First, we found that the expression of FGFR3-AS1 was upregulated about threefold in HCC samples and cell lines. We knocked down FGFR3-AS1 in Huh7 and Hep3B cells and found that FGFR3-AS1 knockdown significantly inhibited cell proliferation but induced apoptosis. Moreover, FGFR3-AS1 knockdown led to more HCC More >

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Haoran Wu¹, Xugang Wang¹, Naixin Mo, Liang Zhang, Xiaoliang Yuan, Zhong Lü
Oncology Research, Vol.26, No.8, pp. 1267-1274, 2018, DOI:10.3727/096504018X15172227703244
Abstract B7-homolog 4 (B7-H4), a member of the B7 family of costimulatory molecules, has been reported to be upregulated in urothelial cell carcinoma. This study was conducted to explore the biological role of B7-H4 in the aggressiveness of bladder cancer and the associated molecular mechanism. We found that the mRNA and protein levels of B7-H4 were significantly greater in bladder cancer cell lines than in SV-HUC-1 (normal human urothelial cells). Overexpression of B7-H4 significantly promoted bladder cancer cell migration and invasion, whereas knockdown of B7-H4 exerted an opposite effect. However, the growth of bladder cancer cells More >

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Ning Wang^*1, Yang Zhang^†1, Huaxin Liang^‡
Oncology Research, Vol.26, No.8, pp. 1275-1283, 2018, DOI:10.3727/096504018X15185735627746
Abstract The dysregulation of microRNA (miRNA) expression is closely related with tumorigenesis and tumor development in glioblastoma (GBM). In this study, we found that miRNA-598 (miR-598) expression was significantly downregulated in GBM tissues and cell lines. Restoring miR-598 expression inhibited cell proliferation and invasion in GBM. Moreover, we validated that metastasis associated in colon cancer-1 (MACC1) is a novel target of miR-598 in GBM. Restoring MACC1 expression reversed the inhibitory effects of miR-598 overexpression on GBM cells. In addition, miR-598 overexpression suppressed Met/ AKT pathway activation in GBM. Our results provided compelling evidence that miR-598 serves More >

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Wei Li, Weidong Zhao, Zhaohui Lu, Wen Zhang, Xuan Yang
Oncology Research, Vol.26, No.8, pp. 1285-1294, 2018, DOI:10.3727/096504018X15166193231711
Abstract Long noncoding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) has been revealed to be associated with the progression of various cancers. However, the biological roles of GAS5 in esophageal cancer (EC) remain unclear. We aimed to thoroughly explore the functions of GAS5 in EC. The results showed that GAS5 expression was increased in EC cells (ECA109, TE-1, TE-3, and EC9706) compared to SHEE cells. Knockdown of GAS5 decreased cell viability, migration, and invasion and induced apoptosis in EC9706 cells. Moreover, miR-301a appeared to be directly sponged by GAS5, and miR-301a suppression obviously alleviated the protumor More >

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Feng Shuai^*, Bo Wang^†, Shuxiao Dong^‡
Oncology Research, Vol.26, No.8, pp. 1295-1305, 2018, DOI:10.3727/096504018X15172747209020
Abstract Among all of the miRNAs, miR-204 has gained considerable attention in the field of cancer research. This study aimed to reveal the detailed functions and the underlying mechanism of miR-204 in colorectal cancer (CRC) cells. The expressions of miR-204 in CRC tumor tissues and cell lines were monitored. Expressions of miR-204 and CXCL8 in Caco-2 and HT-29 cells were altered by transfection, and then cell viability, apoptosis, migration, invasion, EMT-related protein expression, and PI3K/AKT/mTOR pathway protein expression were assessed. We found that miR-204 was expressed at low levels in CRC tumor tissues and cell lines… More >

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